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We narrowed to 13,911 results for: LEA

Showing: 3421 - 3440 of 13911 results

Results per page:

pAAV-ef1a-DIO-eGCaMP(2+)

Plasmid

#201150

Purpose

AAV virus production for Cre recombinase dependent expression of eGCaMP(2+)

Depositor

Andre Berndt

Article

Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

Insert

eGCaMP_2+

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLenti-dSaCas9-KRAB-gRNA-TRE-blast

Plasmid

#201151

Purpose

Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

Depositor

Moshe Szyf

Inserts

SadCas9-KRAB
gRNA-TRE

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mutation

gRNA sequence: ATCAGTGATAGAGAACGTATG

Promoter

Availability

Academic Institutions and Nonprofits only

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pLenti-dCas9-KRAB-gRNA-TRE-blast

Plasmid

#201152

Purpose

Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

Depositor

Moshe Szyf

Inserts

dCas9-KRAB
gRNA-TRE

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mutation

gRNA sequence: TACGTTCTCTATCACTGAT

Promoter

Availability

Academic Institutions and Nonprofits only

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pACS129

Plasmid

#204594

Purpose

Histone H3.1-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

histone H3.1 (H3C3 Human)

Use

Lentiviral

Tags

human histone H3.1-V5

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pET28a_SUMO_AcCas12n

Plasmid

#203808

Purpose

pET28a-based plasmid containing a T7-driven His6-SUMO-HRV3c-AcCas12n cassette. Kanamycin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

SUMO_AcCas12n

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pRegev_V1

Plasmid

#204143

Purpose

Single reporter randomly selected from library for biochemical validation

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

V1 exon in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Truncated CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pRegev_V2

Plasmid

#204144

Purpose

Single reporter randomly selected from splicing library for biochemical validation.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

V2 exon in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Truncated CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pRegev_V3

Plasmid

#204145

Purpose

Single reporter randomly selected from splicing library for biochemical validation

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

V3 exon in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Truncated CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pRegev_S1.46G>U

Plasmid

#204160

Purpose

pRegev_S1 with single mutation that disrupts secondary structure.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

S1 exon with 46G>U substitution in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

46G>U substitution

Promoter

Truncated CAG

Availability

Academic Institutions and Nonprofits only

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pRegev_S1.34C>A_46G>U

Plasmid

#204161

Purpose

pRegev_S1 with double mutations that restore secondary structure.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

S1 exon with 34C>A and 46G>U substitutions in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

34C>A and 46G>U mutations

Promoter

Truncated CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pRegev_S2.39U>G

Plasmid

#204163

Purpose

pRegev_S2 with single mutation that disrupts secondary structure.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon S2 with 39U>G mutation in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

39U>G mutation

Promoter

Truncated CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pRegev_S2.39U>G.51A>C

Plasmid

#204165

Purpose

pRegev_S2 with double mutations that restore secondary structure.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon S2 with 39U>G and 51A>C mutations in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

39U>G and 51A>C mutations

Promoter

Truncated CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pRegev_S4.39U>G

Plasmid

#204171

Purpose

pRegev_S4 with single mutation that disrupts secondary structure.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon S4 with 39U>G mutation in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

39U>G mutation

Promoter

Truncated CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pRegev_S4.51A>C

Plasmid

#204172

Purpose

pRegev_S4 with single mutation that disrupts secondary structure.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon S4 with 51A>C mutation in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

51A>C mutation

Promoter

Truncated CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pRegev_S4.39U>G.51A>C

Plasmid

#204173

Purpose

pRegev_S4 with double mutations that restore secondary structure.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon S4 with 39U>G and 51A>C mutations in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

39U>G and 51A>C mutations

Promoter

Truncated CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pRegev_S5.49C>A

Plasmid

#204175

Purpose

pRegev_S5 with single mutation that disrupts secondary structure.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon S5 with 49C>A mutation in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

49C>A mutation

Promoter

Truncated CAG

Availability

Academic Institutions and Nonprofits only

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pRegev_S5.49C>A.59G>U

Plasmid

#204177

Purpose

pRegev_S5 with double mutations that restore secondary structure.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon S5 with 49C>A and 59G>U mutations in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

49C>A and 59G>U mutations

Promoter

Truncated CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pRegev_D1

Plasmid

#204178

Purpose

Single reporter with long guanine depleted (G-poor) region from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D1 in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Truncated CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pRegev_D2

Plasmid

#204180

Purpose

Single reporter with long guanine depleted (G-poor) region from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D2 in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

61C>G mutation

Promoter

Truncated CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pRegev_D4

Plasmid

#204184

Purpose

Single reporter with long guanine depleted (G-poor) region from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

D4 exon in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Truncated CAG

Availability

Academic Institutions and Nonprofits only

Enlarge