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Plasmid#124440PurposePlasmid for anti-sense in situ probe in vitro transcriptionDepositorInsertGLI-Kruppel family member GLI3 (Gli3 Mouse)
UseIn situ probeTagsExpressionMutationPromoterT7Available sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.EFS.SpCas9.P2A.BSD
Plasmid#57821PurposeLentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
CARPID dCasRx-BASU
Plasmid#153303Purposeexpress CARPID dCasRx-BASU fusion protein in mammalian cellsDepositorInsertdCasRx-BASU
UseCRISPR and LentiviralTagsExpressionMammalianMutationR239A/H244A/R858A/H863A in RfxCas13dPromoterEF-1aAvailable sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CIB1-mCerulean-MP
Plasmid#58366PurposeFor use in light-induced protein inactivation through the interaction with CRY2DepositorUseTagsmCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.EFS.SpCas9.P2A.PAC
Plasmid#58329PurposeLentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
P-rTetO-mCherry-T2A-RPL22-1xV5
Plasmid#170325PurposeTetracycline-inducible expression of V5 and mCherry tagged RPL22DepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab27B N133I
Plasmid#89449PurposeExpression of GFP-tagged human Rab27B N133I in mammalian cellsDepositorInserthuman Rab27B N133I (RAB27B Human)
UseTagseGFPExpressionMammalianMutationN133IPromoterCMVAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
R619-M91-303: CMV51p> HCoV-NL63 S-2P-T4f-3C-His8-Strep2x2
Plasmid#166017Purposemammalian expression of HCoV-NL63 soluble spike trimer proteinDepositorInsertHCoV-NL63 S (spike)
UseTags3C-His8-Strep2x2ExpressionMammalianMutationPromoterCMV51Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-HNRNPR
Plasmid#38067DepositorInsertHNRNPR heterogeneous nuclear ribonucleoprotein R (HNRNPR Human)
UseTagsFLAG, HA, and HisExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
R619-M90-303: CMV51p> HCoV-229E S-2P-T4f-3C-His8-Strep2x2
Plasmid#166016Purposemammalian expression of HCoV-229E soluble spike trimer proteinDepositorInsertHCoV-229E S (spike) (S )
UseTags3C-His8-Strep2x2ExpressionMammalianMutationPromoterCMV51Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Tox2-IRES-eGFP
Plasmid#165428PurposeExpresses TOX2 in mammalian cellsDepositorInsertTOX2 (Tox2 Mouse)
UseTags3xflag 5' of Tox2ExpressionMammalianMutationPromoter5' LTRAvailable sinceMarch 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Neo
Plasmid#170321PurposeExpresses HA and mCherry tagged RPL22, neomycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XFlag-P2A-mCherry-T2A-Puro
Plasmid#170322PurposeExpresses FLAG and mCherry tagged RPL22, puromycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {hCAR}off-{hM4Di-mCherry}on-W3SL
Plasmid#111397PurposeAAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON hM4Di-mCherry (Gi-coupled DREADD for neuronal silencing), W3SL cassette (for maximize cloning capacity)DepositorInsertsUseAAVTagsMyc and mCherryExpressionMammalianMutationPromoterhSynapsinAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NOPLight-ctr
Plasmid#195579PurposeExpresses the control sensor NOPLight-ctr in mammalian cellsDepositorInsertNOPLight-ctr
UseTagsExpressionMammalianMutationD110A, D130APromoterCMVAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPACE (pRZ1813)
Plasmid#140242PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFPDepositorInsertSPACE
UseTagsExpressionMammalianMutationV82G in TadA*, D10A in Cas9, R187W in pmCDA1PromoterCMVAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CIB1-mRFP1-MP
Plasmid#58367PurposeFor use in light-induced protein inactivation through the interaction with CRY2DepositorUseTagsmRFP1ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lenti-DIO-P-Ef1a-mCherry-T2A-RPL22-1xV5
Plasmid#170328PurposeDouble-floxed inverse Orientation V5 and mCherry tagged RPL22DepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat3-SH2
Plasmid#46513DepositorInsertStat3 (STAT3 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 584-700PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti-DIO-P-EF1a-RPL22-3xHA-IRES-YFP
Plasmid#170327PurposeDouble-floxed inverse Orientation HA and IRES-YFP tagged RPL22DepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-EGFP MASTL WT (siRNA resistant)
Plasmid#191011PurposeExpresses EGFP-tagged MASTL WT with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)DepositorInsertMicrotubule-associated serine/threonine kinase like (MASTL Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-BFP
Plasmid#137129PurposeIntersectional viral expression of BFP in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-BFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Foff 2.0-EYFP
Plasmid#137162PurposeIntersectional viral expression of EYFP in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1(-) mouse C/EBP delta
Plasmid#12559DepositorInsertCCAAT/Enhancer-binding Protein delta (Cebpd Mouse)
UseTagsExpressionMammalianMutationS2G introduced during cloningPromoterAvailable sinceJuly 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pACT1:Cas9-GFP, U6:sgTK
Plasmid#122852PurposeExpresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK geneDepositorInsertsCas9-GFP
U6-sgTK
UseCRISPR; Cas9-gfp plasmid for genome editing of cr…TagseGFPExpressionMutationPromoterCryptosporidium parvum U6 and Cryptosporidium par…Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL680 CHYRON3 integration construct
Plasmid#126448PurposeTo integrate the CHYRON3 locus at AAVS1 in human cells.DepositorInsertspU6/3xLacO-CHYRON3 hgRNA
pCMV-puro
UseTagsExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6/3xLacOAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL210
Plasmid#126451PurposeExpresses Cas9-XTEN-TdT in mammalian cells.DepositorInsertCas9-XTEN-TdT (DNTT Human, Streptococcus pyogenes)
UseTagsExpressionMammalianMutationSee depositor comments belowPromoterCMVAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX FynA-SH2
Plasmid#46429DepositorInsertFynA (FYN Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 142-251PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn1-FLEX-mCh-T2A-FLAG-hMOR-WPRE
Plasmid#166970PurposeCre-dependent viral expression of the human mu opioid receptor (hMOR/Oprm1) and mCherry. hMOR contains an N-terminal FLAG tag.DepositorInsertHomo sapiens opioid receptor mu 1 (OPRM1) (OPRM1 Human)
UseAAV and Cre/LoxTagsFLAG, T2A, and mCherryExpressionMammalianMutationPromoterhsynAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-hygro-STING V155M
Plasmid#102599PurposeExpress STING V155M for IFN-beta Luciferase reporter assay.DepositorInsertSTING (STING1 Human)
UseRetroviralTagsExpressionMammalianMutationV155MPromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
YFP-FKBP (YF)
Plasmid#20175DepositorInsertYFP-FKBP (YF): (FKBP1A Human)
UseTagsExpressionMammalianMutationFKBP was taken out from pC4M-F2E with a flanking …PromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
HEpl017
Plasmid#179992PurposepCBh_NLS_TadA8e_dSiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized dSiCas12i-ABE8e driven by CBh promoter and tagmCherry driven by CMV promoterDepositorInsertTadA8e-dSiCas12i(D1049A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pS6-TGFB2-H8
Plasmid#128502PurposeExpresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t H8x tag.DepositorInsertTGFB2 (L21-S414) (TGFB2 Human)
UseTagsHis tag (6x)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
R619-M88-303: CMV51p> MERS-CoV S-2P-T4f-3C-His8-Strep2x2
Plasmid#166013Purposemammalian expression of MERS-CoV soluble spike trimer proteinDepositorInsertMERS-CoV S (spike)
UseTags3C-His8-Strep2x2ExpressionMammalianMutationPromoterCMV51Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-oScarlet
Plasmid#137136PurposeIntersectional viral expression of oScarlet in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationE95DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-muscle Amphiphysin 2
Plasmid#22213DepositorInsertAmphiphysin II (BIN1 Human)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Coff/Fon-ChR2-mCherry
Plasmid#137144PurposeIntersectional viral expression of ChR2-mCherry in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-ChR2-mCherry
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationH134RPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XFlag-P2A-EGFP-T2A-Puro
Plasmid#170318PurposeExpresses FLAG and EGFP tagged RPL22, puromycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STING V155M
Plasmid#102591PurposeLentivector to express STING V155M and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralTagsExpressionMammalianMutationV155MPromoterSFFVAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {mCAR}off-{ChETA-mRuby2}on-W3SL
Plasmid#111390PurposeAAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)DepositorInsertsUseAAVTagsMyc and mRuby2ExpressionMammalianMutationPromoterhSynapsinAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
Plasmid#68345PurposeA Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expressionDepositorInsertspuromycin
hCas9
UseCRISPR; Flp/frtTagslinked to red flouresent protein gene mKateII and…ExpressionMammalianMutationPromoterCAGAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX Fer-SH2
Plasmid#46425DepositorInsertFer (FER Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 450-558PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOTTC385 - pAAV CMV-IE IRES EGFP
Plasmid#102936PurposeAn AAV vector that expresses IRES EGFP under the CMV-IE promoterDepositorInsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterCMV-IEAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-GCaMP6F
Plasmid#137124PurposeIntersectional viral expression of GCaMP6F in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-GCaMP6F
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationRemoved RSET tagPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STING-342STOP
Plasmid#102588PurposeLentivector to express STING delta 342 and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralTagsExpressionMammalianMutationdelta 342PromoterSFFVAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOTTC590 - pCMV ER-iRFP
Plasmid#59136PurposeA plasmid that express endoplasmic reticulum-targeted iRFP under the CMV promoter.DepositorInsertER-localized Infrared Fluorescent Protein
UseTagsER retention signal and signal peptideExpressionMammalianMutationPromoterCMV-IEAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon-iC++-EYFP
Plasmid#137155PurposeIntersectional viral expression of iC++-EYFP in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-iC++-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-Nd1-puro
Plasmid#52052PurposeExpresses Nd1 and puromycin resistent gene under control of TetON promoterDepositorInsertNeurod1 (Neurod1 Mouse)
UseLentiviralTagsT2A-and puromycin resistant cassetteExpressionMammalianMutationPromotertet-onAvailable sinceApril 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDB-hPER2-mScarlet-I-CD4-bla
Plasmid#179449PurposeDonor vector to knock in mScarlet-I C-terminal to human PER2 geneDepositorInsertmScarletI
UseCRISPRTagsHis/FlagExpressionMutationPromoterNoneAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNL-CEF-IgA1
Plasmid#187009PurposeExpression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1DepositorInsertHuman anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only