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Plasmid#212775PurposeC-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with CaUra3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only
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AAV-CamK2-tTA
Plasmid#210731PurposeAAV vector expressing tetracycline-controlled transactivator (tTA) under CamK2 promoter (for expressing tTA in glutamatergic neurons)DepositorInserttetracycline-controlled transactivator (tTA)
UseAAVTagsExpressionMutationPromoterCamK2Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
EC70364
Plasmid#209515PurposeCloning in complete guide expression cassettes, with Cas9DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
EC70197
Plasmid#209458PurposeCloning protospacers for Cas9 editingDepositorInsertCas9 sgRNA
UseCRISPRTagsExpressionMutationPromoterTaU3Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EC70205
Plasmid#209466PurposeCloning protospacers for Cas9 editingDepositorInsertCas9 sgRNA
UseCRISPRTagsExpressionMutationPromoterTaU6Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EC70203
Plasmid#209464PurposeCloning protospacers for Cas9 editingDepositorInsertCas9 sgRNA
UseCRISPRTagsExpressionMutationPromoterTaU6Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRTP801/TA-ODD
Plasmid#206212PurposeAn expression vector of hypoxia-responsive transactivator under control of RTP801 promoterDepositorInserttetracycline-responsive transactivator fused with oxygen-dependent degradation domain
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VEGFR3-V5/HIS
Plasmid#205618PurposeExpression of human VEGFR3 receptor tyrosine kinase in mammalian cellsDepositorInsertVEGFR3 receptor tyrosine kinase (FLT4 Human)
UseTagsV5-HisExpressionMammalianMutationT28M, S455R- Please see depositor commentPromoterCMVAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
GAL-Aur1-2GFP-Hygromycin
Plasmid#184775PurposeYeast expression of Aur1 under GAL promoter. Uses antibiotic resistance marker hphMX6.DepositorInsertproGAL-Aur1 (AUR1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-CRY2PHR-VPR
Plasmid#192608PurposeLentiviral vector for constitutive expression of CRY2PHR-VPR.DepositorInsertCRY2PHR-VPR
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterPGKAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-IDO2-p2a-eGFP
Plasmid#187027PurposeExpresses IDO2 and GFP (seperately) in mammalian cellsDepositorInsertIndoleamine 2,3-dioxygenase 2 (IDO2 Human)
UseTagsP2A-GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7C-DNAJB4
Plasmid#177656PurposeRecombinant protein synthesis of human DNAJB4DepositorInsertDNAJB4 (DNAJB4 Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRhaQCas-BsaI
Plasmid#170631PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
SITS-mCherry-Bin1b
Plasmid#176025PurposeLeishmania cell free expression of zebrafish mCherry-Bin1b. Parton lab clone GSUDepositorInsertBin1b (bin1b Zebrafish)
UseLeishmania cell free expressionTagsmCherryExpressionMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
YSEHPTFTSQY_epitope
Plasmid#165016PurposeExpression of CMV UL83 (363-373) fused to IL-2 signal sequenceDepositorInsertCMV UL83 (363-373)
UseLentiviralTagshuman IL2 signal sequence at N-termExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
KCC2G_HUMAN_D0
Plasmid#79711PurposeThis plasmid encodes the kinase domain of KCC2G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKCC2G (CAMK2G Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KCC2B_HUMAN_D0
Plasmid#79718PurposeThis plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKCC2B
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabePuro-DNAJA4CDS
Plasmid#43799DepositorInsertDNAJA4 CDS (DNAJA4 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceMarch 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-miniTurbo-L2
Plasmid#127352Purposegateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and YFPExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTECH-chPylRS(IPYE)
Plasmid#99222PurposeExpression plasmid encoding chPylRS(IPYE) and pylTDepositorInsertPlpp chPyl(IPYE)RS; PproK pylT
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EML4-ALK variant 3a
Plasmid#183829PurposeExpress EML4-ALK fusion variant 3a (E6a;A20) in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-6a and ALK exon…ExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
nuclear NAD+ biosensor
Plasmid#186789PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleusDepositorInsertnuclear NAD+ biosensor
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
CN4084-pAAV-mMbp_promoter-SYFP2-P2A-3XFLAG-10aa-H2Bz
Plasmid#208160PurposeDirect-expressing SYFP2 in oligodendrocytes AAV VirusDepositorInsertSYFP2
UseAAVTagsExpressionMutationNAPromoterBeta Globin minimal promoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
CaV1.3e[8a,11,31b,Δ32,42a]
Plasmid#49333PurposeRepaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].DepositorInsertCACNA1D (Cacna1d Rat)
UseTagsExpressionMammalianMutationContains exons 8a, 11, 31b and 42a.Deletion of ex…PromoterCMVAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
FPR2-Tango
Plasmid#66284PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertFPR2 (FPR2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-21a_3xNLS_SpCas9_protein_expression
Plasmid#114365PurposeProtein expression plasmid for 3xNLS SpCas9 in pET-21a backboneDepositorInsert3xNLS_SpCas9
UseCRISPRTagsNLSExpressionBacterialMutationPromoterT7Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
N-WASP(CRIB)-mScarlet-I-N-WASP(CRIB)
Plasmid#191451PurposeVisualizing endogenous & active Cdc42DepositorInsertN-WASP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-LC3
Plasmid#21073PurposeMammalian expression of rat LC3 fused to EGFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagsEGFPExpressionMammalianMutationFirst ATG is not included.PromoterCMVAvailable sinceMay 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-STOPS-NLS
Plasmid#175007PurposeNuclear-targeted Akt-STOPS.DepositorInsertmCherry-Akt-STOPS-NLS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K29
Plasmid#22903DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK29 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV mCherry-GFP-LC3B WT
Plasmid#123230PurposeExpresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE30 PTBP1 isoform1
Plasmid#108591PurposeRecombinant His tagged protein productionDepositorInsertPTBP1 isoform 1 (PTBP1 Human)
UseTags6xHisExpressionBacterialMutationisoform 1 (isoform c)PromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
Plasmid#129632PurposeVisualization of Akt and ERK activityDepositorInsertPB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
miniCMV-tdTomato-tDeg
Plasmid#217521PurposeExpresses tdTomato-tDeg fluorogenic protein in mammalian cells with miniCMV promoterDepositorInserttdTomato-tDeg fluorogenic protein
UseTagsExpressionMammalianMutationPromoterminiCMVAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE3G-NFIA+SOX9
Plasmid#129455PurposeInducible overexpression of NFIA and Sox9 in human cells.DepositorInsertSox 9 (SOX9 Human)
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TS2126 RNA Ligase
Plasmid#119941PurposeExpress TS2126 RNA ligase in E. coliDepositorInsertTS2126 RNA ligase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-HA-humanCMYC
Plasmid#74164PurposeMammalian expression of human cMycDepositorInsertcMyc (MYC Human)
UseTags2x HAExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Brainbow3.2-PGK-PuroTK
Plasmid#159928PurposeBrainbow transgenesisDepositorInsertsNFPnls
mOrange2
EGFP
mKate2
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
BB3cK_pGAP_23*_pTEF_Cas9
Plasmid#104909PurposehCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selectionDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEM040 [Multiome Perturb-seq sgRNA expression vector]
Plasmid#224898PurposeLentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experimentsDepositorInsertseGFP
LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only