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Plasmid#226252PurposeEntry plasmid of N-terminally HA-tagged hBIG2 for Gateway systemDepositorInsertBIG2 (ARFGEF2 Human)
UseEntry vectorTagsHAExpressionMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-dCry-V5His
Plasmid#136241PurposeProtein expression of D. melanogaster (Dm) cytochrome with V5 and His tagsDepositorInsertDmCry
UseTagsHis and V5ExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCheY Tc delta C
Plasmid#13520DepositorInsertcruzain
UseTagsCheYExpressionBacterialMutationTruncated variant: stop codon after Gly212PromoterAvailable sinceMarch 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFast-dCry-V5His
Plasmid#136240PurposeProtein expression of D. melanogaster (Dm) cytochrome with C-terminal V5 and His tagsDepositorInsertDmCry
UseTagsHis and V5ExpressionInsectMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-ZfCry4
Plasmid#136242PurposeProtein expression of Zebrafish (Zf) cytochrome 4 with a C terminal FLAG tagDepositorInsertZfCry4
UseTagsFlagExpressionMammalianMutationD196H- Please see depositor comments belowPromoterAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
saposin B^W
Plasmid#161759PurposeSaposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophanDepositorInsertsaposin B R38_W
UseTagsHis-tagExpressionBacterialMutationchanged Arginine 38 to TryptophanPromoterT7Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
p5E-2.3k-fabp2
Plasmid#84927Purpose–2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vectorDepositorInsert–2.3kb promoter of zebrafish fabp2/ifabp gene (fabp2 Zebrafish)
UseTagsNOExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-21(+)Tm9D8* TrpB
Plasmid#207517PurposeExpression of Tm9D8* TrpB for the synthesis of 7-Aza-L-Tryptophan (7AW) from 7-Azaindole (7AI) and serineDepositorInsertTryptophan synthase β-subunit
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(6/7FTrp)RS
Plasmid#207621PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(4/5FTrp)RS
Plasmid#207620PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-MBP-CI2F
Plasmid#191254PurposeExpresses chymotrypsin inhibitor with an N-terminal MBP fusion proteinDepositorInsertChymotrypsin Inhibitor 2
UseTagsMaltose Binding ProteinExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0808
Plasmid#177082PurposeMoClo Level 1, position 4, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31DepositorInserttryptophan decarboxylase (CrTDC) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0774
Plasmid#177064PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInserttryptophan decarboxylase (CrTDC) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0773
Plasmid#177063PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus driven by 35S promoterDepositorInserttryptophan decarboxylase (CrTDC) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDule-pCNF
Plasmid#85494PurposeMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.DepositorInsertpara-cyanophenylalanine Mj synthetase
UseTagsNoneExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-H2B FR-MQV
Plasmid#194079PurposeExpresses nuclear-localized FR-MQV in mammalian cellsDepositorInsertFR-MQV
UseTagsHistone H2BExpressionMammalianMutationMethionine 42 to Glutamine, Cystiene 159 to Valin…PromoterCMV7Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDule2-pCNF
Plasmid#85495PurposeMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.DepositorInsertpara-cyanophenylalanine Mj synthetase
UseTagsNoneExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoterlpp (constitutive)Available sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-UBC-hAAT-IRES-ZsGreen-W
Plasmid#126702Purposelentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreenDepositorInsertAlpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)
UseLentiviralTagsExpressionMutationPromoterUBCAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
tdTomato/pTREX-b
Plasmid#68709PurposeExpresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.DepositorInserttdTomato
UseExpression of tdtomato red fluorescence protein i…TagsExpressionMutationPromoterAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
KG#797
Plasmid#110916PurposeExpresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neuronsDepositorInsertsunc-129 promoter
STRN-1 (Sentryn)-GFP
unc-54 3' control region with 1 intron just upstream and 1 intron in control region
UseTagsGFP (contains 3 artificial introns)ExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only