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Plasmid#58180PurposeLocalization: Cytosolic FRET, Excitation: , Emission:DepositorTypeEmpty backboneUseTagsmCerulean3 and mVenusExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only
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pME-H2B-mCerulean3
Plasmid#75156PurposeNuclear mCerulean2 (blue) fluorophore for use with 5’ promoters.DepositorInsertH2B-mCerulean3
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ccdB-Cerulean
Plasmid#14396DepositorTypeEmpty backboneUseGateway destinationTagsCeruleanExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MCU-mCerulean
Plasmid#185809PurposeHuman mitochondrial calcium uniporter with C-terminus mCerulean tagDepositorInsertMitochondrial calcium uniporter (MCU Human)
UseLentiviralTagsmCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mitoX4-mCerulean
Plasmid#185807PurposeMitochondrial targetted (COX8) mCeruleanDepositorInsertmito-mCerulean
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG425GPD-Cerulean-ccdB
Plasmid#14418DepositorTypeEmpty backboneUseGateway destinationTagsCeruleanExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-PCNA-19-SV40NLS-4
Plasmid#55386PurposeLocalization: Nucleus, Excitation: 433, Emission: 475DepositorInsertPCNA-19 SV40NLS (PCNA Human)
UseTags3xSV40 NLS and mCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCerulean-N1 Cyclin B1 (1147)
Plasmid#61852Purposemammalian expression of Cyclin B1 fused to CeruleanDepositorInsertCyclin B1 (CCNB1 Human)
UseTagsCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-Cerulean-SKL
Plasmid#194048PurposeTransient expression of Cerulean-SKL in plant cell (Peroxisome)DepositorInsertCerulean
UseTagsSer-Lsy-LeuExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOpen-Endonuclease IV E. coli
Plasmid#165551PurposeDNA AP endonuclease; Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini; Also has 3'-diesterase activity which can remove 3' phosphate, 3'-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups.DepositorInsertEndonuclease IV E. coli
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCerulean-Beta-Catenin-20
Plasmid#55352PurposeLocalization: Tight Junctions, Excitation: 433, Emission: 475DepositorInsertBeta-Catenin (Ctnnb1 Mouse)
UseTagsmCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCK036 desmaMCS:minCerulean,cryaa:Venus
Plasmid#195981PurposeExpression constructDepositorInsertdesmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, cryaa Venus (desma Zebrafish)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-Gamma-Tubulin-17
Plasmid#55370PurposeLocalization: Centrosomes, Excitation: 433, Emission: 475DepositorInsertGamma-Tubulin (TUBG1 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEft-3::Cerulean-Venus
Plasmid#68048PurposeUbiquitious expression of oxCerulean-oxVenus (dFP)DepositorInsertpEft-3::Cerulean-Venus
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-Cerulean-SEC4
Plasmid#18844DepositorInsertSEC4 (SEC4 Budding Yeast)
UseTagsCeruleanExpressionYeastMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Bcl2-pEGFP-C1
Plasmid#177416PurposeTo express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2.DepositorInsertBcl-2 (BCL2 Human)
UseTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
AfrLEA3m_mCh-2xFKBP (pBS1137)
Plasmid#185321PurposeFor the mammalian expression of the brine shrimp protein AfrLEA3m attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertAfrLEA3m
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Mcl-1-pLVX
Plasmid#166752PurposeExpress mCerulean3 fused to the N-terminus of Bcl-2 family protein, Mcl-1, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.DepositorInsertMcl-1 (MCL1 Human)
UseLentiviralTagsmCerulean3ExpressionMutationPromoterCMVAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT2-4xUAS:P2A-mCerulean3
Plasmid#127592PurposeThis vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mCerulean3 through P2A self-cleaving peptides.DepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cerulean-WW-NES
Plasmid#84629PurposeEncodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targetedDepositorInsertCerulean-WW-NES
UseTags6xHis, Cerulean, Nuclear export signal (NES), T7 …ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only