Showing: 321 - 340 of 663 results
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Plasmid#111585PurposeAn AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.DepositorInsertmyriRFP670V5-P2A-post-eGRASP
UseAAVTagsExpressionMutationPromoterEf1aAvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Tcf7l1-g1)-PGKpuroBFP-W
Plasmid#105015PurposeLentiviral gRNA plasmid targeting mouse Tcf7l1 , co-expression of TagBFPDepositorInsertgRNA targeting Tcf7l1 (Tcf7l1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_d1_Ub
Plasmid#170729PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
UseTags6xHis-TEVExpressionBacterialMutationThe C-terminus of OTC was truncated by removing o…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pENTR-eGFP-attP(bxb)-*BsdR
Plasmid#183751PurposeBxb1 landing pad cassette.DepositorInsertloxP-eGFP-attP-Bsd-lox251
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pENTR-mCherry-attP(C31)-*BleoR
Plasmid#183752PurposephiC31 landing pad cassette.DepositorInsertFRT-mCherry-attP-BleoR-F3
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Bxb1-LP-TC
Plasmid#183754PurposeAAVS1 targeting construct with the Bxb1 landing pad cassette.DepositorInsertloxP-eGFP-attP-Bsd-lox257
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
AAVS1-phiC31-LP-TC
Plasmid#183755PurposeAAVS1 targeting construct with the phiC31 landing pad cassette.DepositorInsertFRT-mCherry-attP-BleoR-F3
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Bxb1_AJMA
Plasmid#183760PurposeBxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703DepositorInsertsASAP2f
jRCaMP1b
miRFP703
ASAP2f
UseTagsLck and NESExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBR_attB(bxb)_ccdB_lox
Plasmid#183763PurposeBxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)DepositorInsertccdB
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PB-TetR-tdTomato
Plasmid#212958PurposepiggyBac donor vector containing TetR-tdTomato for random integration by PBaseDepositorInsertTetR-tdTomato
UseMouse Targeting; Piggybase integration (transposo…TagstdTomatoExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PB-LacI-eGFP
Plasmid#213021PurposepiggyBac donor vector containing LacI-eGFP for random integration by PBaseDepositorInsertLacI**-eGFP
UseMouse Targeting; Piggybase integration (transposo…TagseGFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGL3 basic Mut E2
Plasmid#48748PurposeExpression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1)DepositorInsertmPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
UseLuciferaseTagsExpressionMutationMutated E-box2 sequence from CACGTT to GCTAGTPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGL3 Pro Mut E2
Plasmid#48750PurposeExpression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)DepositorInsertmPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
UseLuciferaseTagsExpressionMutationMutated E-box2 sequence from CACGTT to GCTAGTPromoterAvailabilityAcademic Institutions and Nonprofits only -
p.UTA.2.0 miR-27a-3p 3 perfect copies.
Plasmid#82439PurposeDual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p in three copiesDepositorInserthsa-miR-27a-3p (MIR27A Human)
UseTagsExpressionMutationThree copies hsa-miR-27a-3p Complementary region …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pScalps_Puro_mCebpe
Plasmid#79076PurposeExpression of mouse CebpeDepositorInsertCebp epsilon (Cebpe Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pScalps_Puro_mCebpa
Plasmid#79551PurposeExpression of mouse CebpaDepositorInsertCebp alpha (Cebpa Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pScalps_Puro_mTet2 catalytic domain HxD
Plasmid#79611PurposeExpression of catalytically inactive mouse Tet2DepositorInsertTet2 (Tet2 Mouse)
UseLentiviralTagsMycExpressionMutationMutant Tet2 with H1302Y, D1304A substitutions in …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA AKT1
Plasmid#78778PurposeTo overexpress AKT1 in Mammalian CellsDepositorInsertAKT1 (AKT1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA p21
Plasmid#78782PurposeTo overexpress p21 in Mammalian CellDepositorInsertp21 (CDKN1A Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro p21
Plasmid#78783PurposeRetroviral expression of p21DepositorInsertp21 (CDKN1A Human)
UseRetroviralTagsExpressionMammalianMutationPromoter5'LTRAvailabilityAcademic Institutions and Nonprofits only
Showing: 321 - 340 of 663 results