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Plasmid#133996PurposeEnv gene from HIV-1 LAI in pcDNA3.1 expression vectorDepositorInsertEnvelope Glycoprotein
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac1.8
Plasmid#119871PurposeConstitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR'
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationSNP in 592 bpPromoterPserA promoter and Pveg promoter with CuO sequenceAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE3GS-->mCherry_PGK-->BFP
Plasmid#162231PurposeLentiviral vector for the expression of TRE3GS-->mCherry_PGK-->BFP in mammalian cellsDepositorInsertmCherry and BFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagD 77L
Plasmid#19308DepositorInsertRas-related GTP binding D (RRAGD Human)
UseTagsGST and HAExpressionMammalianMutationS77LPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagB 99L
Plasmid#19303DepositorInsertRas-related GTP binding B (RRAGB Human)
UseTagsGST and HAExpressionMammalianMutationQ99LPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pT2/shp53/GFP4
Plasmid#20208DepositorInsertshp53/GFP4
UseTransposonTagsExpressionMutationPromoterAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STING
Plasmid#102586PurposeLentivector to express STING and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
mouse E-cadherin GFP (423)
Plasmid#67937PurposeMammalian expression of E-cadherin-GFPDepositorInsertE-cadherin (Cdh1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
HDAC5 Flag
Plasmid#13822PurposeMammalian expression of human histone deacetylase 5 with flag tagDepositorInsertHDAC5 (HDAC5 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
CMV-ER-LAR-GECO1
Plasmid#61244PurposeExpresses LAR-GECO1 in the endoplasmic reticulum in mammalian cellsDepositorInsertLAR-GECO1
UseTagsER-retention sequence: KDEL and ER-targeting sequ…ExpressionMammalianMutationSubstitutions relative to R-GECO1: V51W/I113V/N35…PromoterCMVAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHIPH4
Plasmid#117685PurposeHansenula polymorpha expression plasmidDepositorInsertAlcohol Oxidase promoter
UseTagsExpressionYeastMutationPromoterAlcohol OxidaseAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
PEmax-mRNA
Plasmid#204472Purposeplasmid for PEmax mRNA IVTDepositorInsertPrime editor
UseCRISPRTagsnoExpressionMammalianMutationPromoterT7Available sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3363
Plasmid#144839PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNR5A1 (NR5A1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-BP
Plasmid#60496PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and puromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
35S-GAL4BD-GWY/pAM
Plasmid#201233PurposeUsed to produce and express in planta N-terminal fusion proteins with the GAL4 BDDepositorInsert35S-GAL4BD-GWY
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-cGASsh4
Plasmid#127646PurposeKnock-down of human cGASDepositorInsertcGAS shRNA (CGAS Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSH-CAG-AtAFB2.F74A-IRES-puro
Plasmid#216245PurposeExpress AtAFB2.F74A mutant without tag for AID2DepositorInsertAtAFB2.F74A
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsExpressionMammalianMutationF74A mutationPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas
Plasmid#106171Purposecre mediated red to green shiftDepositorInsertsdsRED-express2
eGFP
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrEno
Plasmid#115474PurposeEno-promoter driven expression vector for protein production in Trichoderma reeseiDepositorInsertCel7A
UseUnspecifiedTagsExpressionMutationIntrons have been removedPromoterTrichoderma reesei Enolase (Eno) promoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
p201N Cas9
Plasmid#59175PurposeCas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
nptII
UseCRISPRTagsExpressionMutationPromoter2x35S and StUbi-3PAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
JD553
Plasmid#160392PurposeWheat GRF4-GIF1 chimera in pDONR-zeoDepositorInsertwheat GRF4-GIF1 chimera
UseEntry clonTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLY005SB_pAAV-U6sg(BbsI)-EFS-SB100X
Plasmid#192148PurposeT cell CRISPR AAV vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationNAPromoterAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-STING-sh5
Plasmid#127647PurposeKnock-down of human STINGDepositorInsertSTING shRNA (STING1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct-miRFP670nano
Plasmid#127434PurposeF-Actin labeling with near-infrared fluorescent protein miRFP670nanoDepositorInsertLifeAct-miRFP670nano
UseTagsmiRFP670nanoExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR(T/A)
Plasmid#64928PurposeNegative-control mutant for TORCAR biosensor.DepositorInsertTORCAR(T/A) (EIF4EBP1 Synthetic, Human)
UseTagsCerulean and YPetExpressionMammalianMutation4EBP1 domain contains T37A and T46A mutations.PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJH145
Plasmid#68380PurposeIMCE attB donor copy control fosmidDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBMN-I-GFP
Plasmid#1736PurposeThis retroviral vector has an IRES-GFP downstream of the cloning sitesDepositorInsertIRES-GFP
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FlagHA_USP10_C424A
Plasmid#127103DepositorInsertUSP10 (USP10 Human)
UseTagsExpressionMammalianMutationC424APromoterAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDR111_Bgal no NLS
Plasmid#188397PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
UseTagsPhoD secretion peptideExpressionBacterialMutationPromoterPhyper spankAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVTRE3G eGFP Puro (w819-1)
Plasmid#27570DepositorInsertEnhance Green Fluorescent Protein
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
TFORF2155
Plasmid#141948PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertBCL11A (BCL11A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-puro-PTPIP51(1-470)
Plasmid#170536PurposeExpresses PTPIP51(1-470) in human HeLa cells after transfectionDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterCAG promoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA Sec13 pRK5
Plasmid#46332DepositorInsertSec13 (SEC13 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5 bovine GRK2
Plasmid#14691DepositorInsertGRK2 (GRK2 Bovine)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMRE145
Plasmid#118497Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pInt-ts
Plasmid#66076PurposeThe pInt-ts plasmid [Haldimann2001] is the lambda helper plasmid necessary for the genome integration of the burden monitor lambda versionDepositorInsertlambda integrase
UseTagsExpressionMutationPromoterAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP×3-N1
Plasmid#86776PurposeA mammalian expression vector with triple EGFP at C-terminus.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Nst-CAG-DEST
Plasmid#80489PurposeGateway Destination donor vector for AAVS1 targeting (puromycin selection)DepositorTypeEmpty backboneUseDonor vector (human)TagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFD152
Plasmid#125546Purposederivative of pFD116 with an optimized RBS for E. coliDepositorInsertoptimized RBS
UseCRISPRTagsExpressionMutationPromoteranhydrotetracycline Ptet promoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
LeGO-Cer2
Plasmid#27338DepositorInsertSFFV promoter, Cerulean
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
PCS4 3XFLAG-PPARgamma1
Plasmid#78769PurposeTo overexpress PPARgamma1 in Mammalian CellsDepositorInsertPPAR_1 (Pparg Mouse)
UseTags3xFLAGExpressionMammalianMutationPromoterCMV IE94Available sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
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piRFP-N3-Tyrosinase
Plasmid#80152PurposeMelanosome markerDepositorInsertTyrosinase (TYR Human)
UseTagsiRFP682ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET251-pUC 12xMS2V6 Loxp KANr Loxp
Plasmid#104392PurposeYeast endogenous mRNA taggingDepositorInsert12xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-LAP2-mCherry
Plasmid#159524PurposeExpresses mCherry from LAP2 promoterDepositorInsertmCherry
UseAAVTagsExpressionMammalianMutationPromoterLAP2Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro
Plasmid#1764PurposeRetroviral vector for cloning and expressing your gene of interest. Puromycin selection.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-PI4KB
Plasmid#139310PurposeRecruitable PI4KBDepositorInsertmCherry: FKBP1A(3-108): GGSA]4GG:PI4KB (PI4KB Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
HDAC8 Flag
Plasmid#13825DepositorInsertHDAC8 (HDAC8 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCas9-sgAAVS1-2
Plasmid#129727PurposeExpressing SpCas9 and sgAAVS1-2DepositorInsertSpCas9 and sgAAVS1-2
UseCRISPRTagsT2A-mCherry-P2A-PuroExpressionMammalianMutationPromoterhU6Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Electra2-P2A-EGFP
Plasmid#184936PurposeExpresses blue fluorescent protein Electra2 and EGFP in mammalian cellsDepositorInsertElectra2-P2A-EGFP
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only