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Plasmid#186370PurposeDestination vector for the expression of a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only
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Avs4_Chimera
Plasmid#188802PurposeChimeric Avs4 with transmembrane effectorDepositorInsertAvs4 chimera
UseTagsExpressionBacterialMutationPromoterEcAvs4 native promoterAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMLS715
Plasmid#188887PurposeMultisite Gateway [4-3] Destination vector; lox2272-flanked Cbr-unc-119 marker; homology to 5' miniMos ArmDepositorTypeEmpty backboneUseMultisite gateway [4-3] destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST tol2 UbB polyA
Plasmid#188701PurposeGateway destination vector containing the zebrafish ubiquitin B polyadenylation sequenceDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Avs4_Archaeon_isolate_HyVt-335
Plasmid#188800PurposeAvs4 from Archaeon isolate HyVt-335DepositorInsertAvs4
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJH026_proD_Cas12c-multiplexing_CAM
Plasmid#183073PurposePlasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repressionDepositorInsertExpressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLucia
Plasmid#135287PurposeGBPart - Lucia Luciferase CDSDepositorInsertLucia Luciferase
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMetLuc
Plasmid#135286PurposeGBPart - MetLuc Luciferase CDSDepositorInsertMetridia Luciferase
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOD_D4800EC
Plasmid#176876PurposeEmpty backbone for Golden Gate cloning into Cermak, et al., 2017 ToolkitDepositorTypeEmpty backboneUseCloningTagsExpressionMutationPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A4800EC
Plasmid#176873PurposeEmpty backbone for Golden Gate cloning into Cermak, et al., 2017 ToolkitDepositorTypeEmpty backboneUseCloningTagsExpressionMutationPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B4800EC
Plasmid#176874PurposeEmpty backbone for Golden Gate cloning into Cermak, et al., 2017 ToolkitDepositorTypeEmpty backboneUseCloningTagsExpressionMutationPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-HsPAN3_245-687-M546FP547G_U
Plasmid#147685PurposeBacterial Expression of HsPAN3_245-687-M546FP547GDepositorInsertHsPAN3_245-687-M546FP547G (PAN3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-DmPAN3_349-790-P652G_T
Plasmid#147570PurposeBacterial Expression of DmPAN3_349-790-P652GDepositorInsertDmPAN3_349-790-P652G (CG11486 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
BASIC_SEVA_36.10
Plasmid#178924PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
BASIC_SEVA_39.10
Plasmid#178926PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
BASIC_SEVA_35a.10
Plasmid#178923PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
BASIC_SEVA_37.10
Plasmid#178925PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEc-crRNA2
Plasmid#170089Purposeencodes E. coli type I-E single spacer arrayDepositorInsertE. coli type I-E single spacer array
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterJ23119Available sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
PROM5_Ntprrn_rev_chlo
Plasmid#163952PurposeL0 part - promoterDepositorInsertfrom GenBank: KT364744.3
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PROM5_Ntpsba_chlo
Plasmid#163953PurposeL0 part - promoterDepositorInsertfrom GenBank: KT364744.3
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits