pFBXC3H
(Plasmid
#49050)
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Purpose(Empty Backbone) FX cloning insect cell expression vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 49050 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFBDM
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Backbone manufacturerImre Berger
- Backbone size (bp) 6992
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Modifications to backbonemodified for compatibility with FX cloning and added tags/fusion proteins
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Vector typeInsect Expression
- Promoter polH (polyhedrin)
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Tags
/ Fusion Proteins
- 3C protease site (PreScission site) (C terminal on backbone)
- decaHis (10x His) (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Copy numberLow Copy
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer polyhedrin-fwd
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Constructed by Christopher Weinert, Grütter lab, Dept of Biochemistry, University of Zurich, Switzerland.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFBXC3H was a gift from Markus Gruetter (Addgene plasmid # 49050 ; http://n2t.net/addgene:49050 ; RRID:Addgene_49050) -
For your References section:
Crystal structure of TRIM20 C-terminal coiled-coil/B30.2 fragment: implications for the recognition of higher order oligomers. Weinert C, Morger D, Djekic A, Grutter MG, Mittl PR. Sci Rep. 2015 Jun 4;5:10819. doi: 10.1038/srep10819. 10.1038/srep10819 PubMed 26043233