...General protein expression BL21 (DE3) pLysS* Chloramphenicol (pLysS) pLysS expresses T7 lysozyme to reduce...Expression of toxic proteins BL21 (DE3) pLysE* Chloramphenicol (pLysE) pLysE has higher T7 lysozyme expression...Expression of insoluble proteins  Rosetta2 (DE3)* Chloramphenicol (pRARE) Good for “universal” translation; contains...Expression of eukaryotic proteins Lemo21 (DE3)* Chloramphenicol (pLemo) Rhamnose-tunable T7 RNAP expression...T7 expression. The pLys plasmid contains a chloramphenicol resistance cassette for positive selection ...

...protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol 48650 PM-SP!TB Bacterial SP crRNA expression...protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol 48651 PM-NM!TA Bacterial NM crRNA expression...protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol 48652 PM-NM!TB Bacterial NM crRNA expression...protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol 48653 PM-ST1!TA Bacterial ST1 crRNA expression...protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol 48654 PM-ST1!TB Bacterial ST1 crRNA expression...protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol 48655 PM-TD!TA Bacterial TD crRNA expression...protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol 48656 PM-TD!TB Bacterial TD crRNA expression...

...exchange vector. This vector contains sacB, an chloramphenicol resistance cassette, and the R6K origin of ...products. To allow manipulation of naturally chloramphenicol-resistant strains, we have also included versions...

...elongation step) Bacteriostatic 10 ug/mL Chloramphenicol   Binds 50S ribosomal subunit; inhibits peptidyl...

...amplifying more copies with the addition of chloramphenicol – make sure your growth conditions aren't working...

...reporter gene has over the years evolved from chloramphenicol acetyltransferase to luciferase, the basic ...

... apramycin, blasticidin, bleocin (zeocin), chloramphenicol, erythromicin, gentamicin, hygromycin, kanamycin...

...e.g. p15A, ColE1) can be increased by adding chloramphenicol to the culture medium. Improve the culture ...

...Marraffini pCas9 BsaI E. coli, S. pneumoniae Chloramphenicol yes, cut Marraffini pgRNA-bacteria BBa_J23119...

...inducible dcas9 gene on a p15A vector with chloramphenicol resistance. The second plasmid ( pgRNA_bacteria...

...base editing. They first created a defective chloramphenicol resistance gene (CamR) by introducing a point...

...hsdR17(rK- mK+)  XL10 Gold Tetracycline and Chloramphenicol High competency cloning and propagation of ...

...mL Bleocin 5 µg/mL Carbenicillin 100 µg/mL Chloramphenicol 25 µg/mL Coumermycin 25 µg/mL Gentamycin 10...

...pyogenes Chloramphenicol Marraffini pCas9 42876 Bacteria BsaI yes, cut S. pyogenes Chloramphenicol Marraffini...

... of two plasmids: pCas9: carries Cas9 and chloramphenicol resistance pCRISPR: carries a spacer targeting...

...5 µg/mL Carbenicillin* 100 mg/mL 100 µg/mL Chloramphenicol 25 mg/mL (dissolve in EtOH) 25 µg/mL Coumermycin...

...primer CAT-R GCAACTGACTGAAATGCCTC 5' end of chloramphenicol resistance gene, reverse primer CMV Forward...

...100 µg/mL Carbenicillin* 100 mg/mL 100 µg/mL Chloramphenicol 25 mg/mL (dissolve in EtOH) 25 µg/mL Hygromycin...