Plasmids 101: E. coli Strains for Protein Expression
Type
Blog Post
Published
Feb. 10, 2015, 3:06 p.m.
...General protein expression
BL21 (DE3) pLysS*
Chloramphenicol (pLysS)
pLysS expresses T7 lysozyme to reduce...Expression of toxic proteins
BL21 (DE3) pLysE*
Chloramphenicol (pLysE)
pLysE has higher T7 lysozyme expression...Expression of insoluble proteins
Rosetta2 (DE3)*
Chloramphenicol (pRARE)
Good for “universal” translation; contains...Expression of eukaryotic proteins
Lemo21 (DE3)*
Chloramphenicol (pLemo)
Rhamnose-tunable T7 RNAP expression...T7 expression. The pLys plasmid contains a chloramphenicol resistance cassette for positive selection ...