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AAV-CAG-DIO-erHRP(N175S mutant)

Plasmid

#79906

Purpose

Adeno-associated virus plasmid, Cre-dependent FLEX switch, erHRP(N175S mutant)

Depositor

Joshua Sanes

Article

Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

Insert

erHRP(N175S mutant)

Use

AAV and Cre/Lox

Tags

HA tag

Expression

Mammalian

Mutation

N175S mutation

Promoter

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo

Plasmid

#112684

Purpose

An AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinase

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

iRFP713

Use

AAV

Tags

2A-Flpo and Nuclear localization signal

Expression

Mutation

Promoter

CMV-IE

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-LMO3 (sbGLuc-VChR1-EYFP)

Plasmid

#114104

Purpose

fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Inserts

Gaussia luciferase, slow burn
Volvox Channelrhodopsin 1
Enhanced Yellow Fluorescent Protein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC351 - pAAV CaMKIIa hChR2(H134R)-iRFP

Plasmid

#47905

Purpose

An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) under the CaM Kinase IIa promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

hChR2(H134R)

Use

AAV

Tags

iRFP

Expression

Mammalian

Mutation

H134R

Promoter

CaMKIIa

Available since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1(D908A)-2A-GFP-U6-AAVS1-sgRNA

Plasmid

#194727

Purpose

CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector, with guide RNA array targeting hAAVS1.

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1(D908A) (AAVS1 Acidaminococcus sp.)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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AAV Cag Flex H2B Gcamp6s reverse

Plasmid

#74157

Purpose

Calcium Sensor

Depositor

Loren Looger

Insert

Gcamp6s

Use

AAV

Tags

H2B

Expression

Mutation

Promoter

SpeI

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-sCreDIO hChR2(H134R)-EYFP

Plasmid

#55642

Purpose

sCre-Dependent ChR2-EYFP

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

hChR2(H134R)-EYFP

Use

AAV; Scre-dependent

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-ChETA-TdTomato-WPRE-pA

Plasmid

#37089

Purpose

Expresses ChETA-tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

ChETA-TdTomato

Use

AAV and Cre/Lox; Cre-off

Tags

TdTomato

Expression

Mutation

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only
AAV-FLEX-tmeGFP-IRES-nls-mRuby2

Plasmid

#63060

Purpose

Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

nls-mRuby2

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-deltaTSR3-CTD-HA.SV40(polyA)

Plasmid

#155194

Purpose

Expresses truncated (AA 1-690) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

Truncated sequence of THBS1 (expresses Amino Acid…

Promoter

CMV

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-LMO4 (GLucM23-VChR1-EYFP)

Plasmid

#114101

Purpose

fusion protein of Gaussia luciferase variant M23, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Park et al J Neurosci Res. 2017 Sep 1. doi: 10.1002

Inserts

Gaussia luciferase, M23
Volvox Channelrhodopsin 1
Enhanced Yellow Fluorescent Protein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC710 - pAAV EF1a DIO Mem-AcGFP

Plasmid

#75081

Purpose

An AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

Insert

Mem-AcGFP

Use

AAV and Cre/Lox

Tags

Palmitylation site

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Linker-hSyn::mCherry.3xFLAG-WPRE

Plasmid

#120391

Purpose

pAAV plasmid expressing mCherry.3XFLAG under the hSyn promoter with a Linker containing BstBI, BspQI and SacI sites upstream of the hSyn promoter

Depositor

Florian Freudenberg

Article

Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

Type

Empty backbone

Use

AAV

Tags

mCherry.3XFLAG

Expression

Mutation

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-T2A-miniSOG-C-Far

Plasmid

#193915

Purpose

Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Farnesylation signal sequence and Flag tag

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-Archon1-KGC-GFP-ER2

Plasmid

#115890

Purpose

AAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version). Using SV40 signal.

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

Promoter

EF1a（1.1kb short version)

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-bREACHes-EYFP

Plasmid

#137147

Purpose

Intersectional viral expression of bREACHes-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Insert

Coff/Fon-bREACHes-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-Egr3-EYFP-WPRE

Plasmid

#186417

Purpose

Overexpression of Egr3 transcript variant 1 CDS under EF-1α promoter

Depositor

Mary Kay Lobo

Article

Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

Insert

Early growth response 3 (Egr3 Mouse)

Use

AAV

Tags

EYFP

Expression

Mutation

Promoter

EF-1-alpha

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-NpHR3.0-eYFP-WPRE-pA

Plasmid

#37088

Purpose

Expresses NpHR3.0-EYFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic inhibition

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

NpHR3.0-EYFP

Use

AAV and Cre/Lox; Cre-off

Tags

EYFP

Expression

Mutation

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-NES-tdTomato-LINuS-WPRE

Plasmid

#159894

Purpose

AAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signal

Depositor

Tatsushi Onaka

Article

Inutsuka et al Biochem Biophys Res Commun. 2020 Jan 29;

Insert

NES-tdTomato-LINuS

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1α promoter

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-Diras2_1-hSyn::mCherry.3xFLAG-WPRE

Plasmid

#120393

Purpose

pAAV plasmid expressing Diras2 shRNA1 under the U6 promoter and mCherry.3XFLAG under the hSyn promoter

Depositor

Florian Freudenberg

Article

Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

Insert

Diras2 shRNA (Diras2 Mouse)

Use

AAV and RNAi

Tags

mCherry.3XFLAG

Expression

Mutation

Promoter

U6

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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