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Plasmid#212318PurposeExpress MmFnuc with pureexpressDepositorInsertMercenaria
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
mhc2dab:GFP-LT
Plasmid#68996Purposefor making transgenic fishDepositorInsertmhc2dab (mhc2dab Zebrafish)
UseZebrafish expressionTagsGFP-LTExpressionMutationPromotermhc2dabAvailable sinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_g5-HT2m
Plasmid#208710PurposeExpresses the green 5-HT sensor GRAB_g5-HT2m in mammalian cellsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT2m
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_r5-HTmut
Plasmid#208714PurposeExpresses the red 5-HT sensor GRAB_r5-HTmut in mammalian cellsDepositorInsertRed fluorescent 5-HT sensor GRAB_r5-HTmut
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pminiTol2-HuC-NE2m
Plasmid#208688PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m pan-neuronally in zebrafishDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2m
UseCloning vectorTagsExpressionMutationPromoterHucAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_g5-HT3.0mut
Plasmid#208712PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0mut in mammalian cellsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0mut
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
mhc2dab:mCherry
Plasmid#68997Purposefor making transgenic fishDepositorInsertmhc2dab (mhc2dab Zebrafish)
UseZebrafish expressionTagsmcherryExpressionMutationPromotermhc2dabAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S1.46G>U
Plasmid#204160PurposepRegev_S1 with single mutation that disrupts secondary structure.DepositorInsertS1 exon with 46G>U substitution in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation46G>U substitutionPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S2.39U>G
Plasmid#204163PurposepRegev_S2 with single mutation that disrupts secondary structure.DepositorInsertExon S2 with 39U>G mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation39U>G mutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S4.39U>G.51A>C
Plasmid#204173PurposepRegev_S4 with double mutations that restore secondary structure.DepositorInsertExon S4 with 39U>G and 51A>C mutations in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation39U>G and 51A>C mutationsPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S1.34C>A_46G>U
Plasmid#204161PurposepRegev_S1 with double mutations that restore secondary structure.DepositorInsertS1 exon with 34C>A and 46G>U substitutions in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation34C>A and 46G>U mutationsPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S4.51A>C
Plasmid#204172PurposepRegev_S4 with single mutation that disrupts secondary structure.DepositorInsertExon S4 with 51A>C mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation51A>C mutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S4.39U>G
Plasmid#204171PurposepRegev_S4 with single mutation that disrupts secondary structure.DepositorInsertExon S4 with 39U>G mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation39U>G mutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_D1
Plasmid#204178PurposeSingle reporter with long guanine depleted (G-poor) region from splicing library.DepositorInsertExon D1 in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S2.39U>G.51A>C
Plasmid#204165PurposepRegev_S2 with double mutations that restore secondary structure.DepositorInsertExon S2 with 39U>G and 51A>C mutations in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation39U>G and 51A>C mutationsPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S5.49C>A
Plasmid#204175PurposepRegev_S5 with single mutation that disrupts secondary structure.DepositorInsertExon S5 with 49C>A mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation49C>A mutationPromoterTruncated CAGAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_D4
Plasmid#204184PurposeSingle reporter with long guanine depleted (G-poor) region from splicing library.DepositorInsertD4 exon in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTruncated CAGAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_D2
Plasmid#204180PurposeSingle reporter with long guanine depleted (G-poor) region from splicing library.DepositorInsertExon D2 in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation61C>G mutationPromoterTruncated CAGAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR1a-jAspSnFR3-mRuby3-NLS
Plasmid#203489PurposeNuclear directed jAspSnFR3-mRuby3 in Gateway cloning entry vectorDepositorInsertjAspSnFR3-mRuby3-NLS
UseGateway entry vectorTagsNuclear localization signal and mRuby3ExpressionMutationPromoterAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S5.49C>A.59G>U
Plasmid#204177PurposepRegev_S5 with double mutations that restore secondary structure.DepositorInsertExon S5 with 49C>A and 59G>U mutations in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation49C>A and 59G>U mutationsPromoterTruncated CAGAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only