mhc2dab:GFP-LT
(Plasmid #68996)

• Purpose: for making transgenic fish
• Depositing Lab: David Traver
• Publication: Wittamer et al Blood. 2011 Jun 30;117(26):7126-35. doi: 10.1182/blood-2010-11-321448. Epub 2011 Mar 15.

Backbone

• Vector backbone: mTol2-GFP-LT
• Vector type: Zebrafish expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mhc2dab
• Species: D. rerio (zebrafish)
• Insert Size (bp): 3800
• GenBank ID: AL928944.8
• Entrez Gene: mhc2dab (a.k.a. DAB, DAB1, dab1*01, dab101, dab2*01, dab201, dab3*01, dab301, dab4*01, dab4*02, dab401, dab402, mhc2d8.37b3, mhc2deb, si:dkeyp-2h4.1, zgc:110349, zgc:152682)
• Promoter: mhc2dab
• Tag / Fusion Protein:
  • GFP-LT (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: GAGCGGCCGCCTTAGTGTATGTACGAGTGTATAGATGTTTCCC
• 3′ sequencing primer: GAGGATCCGAGTCTTTGAATGTGTCAAATGAAGAACTTTC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A 3.8-kb fragment upstream of the mhc2dab transcriptional start site was amplified from the bacmid AL928944