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FCK-QuasAr2-mRuby2 eFRET

Plasmid

#59174

Purpose

Fluorescent membrane voltage sensor

Depositor

Adam Cohen

Article

Zou et al Nat Commun. 2014 Aug 13;5:4625. doi: 10.

Insert

QuasAr2-mRuby2 eFRET

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-mRuby2

Plasmid

#99117

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mRuby2

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

DIO-mRuby2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pHIS3p:mRuby2-Tub1+3'UTR::TRP1

Plasmid

#50651

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

mRuby2-Tub1

Use

Yeast expression

Tags

mRuby2

Expression

Mutation

Promoter

HIS3p

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pTol2-elavl3_NES-rsCaMPARI-mRuby3

Plasmid

#122129

Purpose

Pan-neuronal expression of rsCaMPARI in zebrafish, nucleus excluded

Depositor

Eric Schreiter

Article

Sha et al Elife. 2020 Sep 15;9. pii: 57249. doi: 1

Insert

rsCaMPARI

Use

Zebrafish expression

Tags

NES-His and mRuby3

Expression

Mutation

Promoter

elavl3

Available since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomRuby-SpHis5

Plasmid

#44843

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mRuby with SpHis5 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomRuby

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pHIS3p:mRuby2-Tub1+3'UTR::HIS3

Plasmid

#50657

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

mRuby2-Tub1

Use

Yeast expression

Tags

mRuby2

Expression

Mutation

Promoter

HIS3p

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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AAV Ef1a-mRuby3-WPRE

Plasmid

#135427

Purpose

Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

Depositor

Rylan Larsen

Insert

mRuby3

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pBS L30 mRuby3-Rab11A

Plasmid

#166430

Purpose

Rab11A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

Depositor

Raphael Gaudin

Insert

Rab11A

Use

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

L30 weak promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pVH232-Tier1-PmPGK1-mRuby2

Plasmid

#169523

Purpose

Tier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven monomeric red fluorescent protein mRuby2

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBS L30 mRuby3-Rab27A

Plasmid

#166432

Purpose

Rab27A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

Depositor

Raphael Gaudin

Insert

Rab27A

Use

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

L30 weak promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pBS L30 mRuby3-Rab5c

Plasmid

#166426

Purpose

Rab5C fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

Depositor

Raphael Gaudin

Insert

Rab5C

Use

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

L30 weak promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mRuby2-T2A-mTurquoise2

Plasmid

#118429

Purpose

Produces equimolar expression of mRuby2 and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mRuby2-T2A-mTurquoise2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Chronos(M140T)-mRuby2-ST

Plasmid

#109129

Purpose

Modified cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

Chronos(M140T)-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-ChroME(F243Y)-mRuby2-ST

Plasmid

#109127

Purpose

Modified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ChroME(F243Y)-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-mRuby-WDR34

Plasmid

#66599

Purpose

Expresses WDR34 as a fusion with monomeric mRuby

Depositor

David Stephens

Article

Asante et al J Cell Sci. 2014 Nov 1;127(21):4774-87.

Insert

WDR34 (WDR34 Human)

Use

Lentiviral

Tags

mRuby

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
CMV-IgK-pHluorin-TM-mRuby

Plasmid

#185546

Purpose

Expresses surface-localized pHluorin fused to intracellular expression of mRuby under CMV promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

IgK-pHluorin-TM-mRuby

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-mRuby3-Pho8-406

Plasmid

#207043

Purpose

Expression of mRuby3-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

PHO8

Use

Tags

mRuby3

Expression

Yeast

Mutation

Promoter

pCUP1

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti7-3_EF1ap-mRuby2_hU6p_sgSTK4i_#1 (BlasR)

Plasmid

#209776

Purpose

CRISPRi for STK4

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

Nluc, sgSTK4i, mRuby2 (STK4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-jAspSnFR3-mRuby3

Plasmid

#203459

Purpose

jAspSnFR3-mRuby3 in adenoviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3

Use

AAV and Adenoviral

Tags

His-tag and mRuby3

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW Syt9 IRES mRuby3

Plasmid

#195700

Purpose

Lentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

encodes only the first 68 amino acids of Tac1

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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