-
Plasmid#61458PurposeExpress pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagyDepositorInsertPK-hLC3 (MAP1LC3B Fission Yeast, Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
DNA-PKcs PQR>ala
Plasmid#83319Purposehuman DNA-PKcs PQR>alaDepositorInserthuman DNA-PKcs (PRKDC Human)
UseTagsExpressionMammalianMutation5 phosphorylation sites in the PQR cluster substi…PromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIRES2Cx32WTEGFP
Plasmid#78150PurposeMammalian Expression of Connexin 32 and EGFP from a bicistronic mRNADepositorInsertConnexin 32 (GJB1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF3126
Plasmid#144602PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertIKZF3 (IKZF3 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
plenti-FLAG-CAMK2D-ED
Plasmid#221700PurposeLentiviral expression of FLAG-tagged human CAMK2D (K43R/D136N) in mammalian cellsDepositorInsertCAMK2D (CAMK2D Human)
UseLentiviralTagsExpressionMutationK43R, D136NPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hMECP2-cycle3GFP
Plasmid#163706PurposepAAV plasmid for Cre-dependent expression of human MECP2 fused with cycle3 GFP under Syn promoterDepositorInserthMECP2-cycle3GFP (MECP2 Aequorea victoria, Human)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer-myc-ERK2-R67S/S153D
Plasmid#213597PurposeDoxycycline inducible expression of myc-tagged ERK2-R67S/S153D cDNA in mammilian cellsDepositorInsertMAPK1 (MAPK1 Human)
UseLentiviralTagsMYCExpressionMammalianMutationR67S, S153DPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hTGM2_C277S-Flag
Plasmid#180412Purposelentiviral transduction of human TGM2 transcript variant 2 with a point mutation (C277S) that inactivate the enzyme and also with a C-terminal Flag tagDepositorInsertTGM2_C277S (TGM2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationC277SPromoterCMVAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
OPRD1-DuET
Plasmid#213358PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertOPRD1 (OPRD1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-DAP5-3XFLAG
Plasmid#127331PurposeExpress 3xFLAG tagged DAP5 protein from AUG start codonDepositorInsertDAP5 (EIF4G2 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-hFGD5
Plasmid#50760Purposeexpression of EGFP-tagged human FGD5DepositorInsertFGD5 (FGD5 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-ERP2-(N-HA)KRAS_G12D
Plasmid#201254PurposeDox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cellsDepositorInsertKRAS (KRAS Human)
UsePiggybac transposonTagsHA-TagExpressionMammalianMutationchanged Gly (G) 12 to Asp (D)PromoterTREAvailable sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MAP3K7
Plasmid#23693DepositorInsertMAP3K7 (MAP3K7 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine
Plasmid#50454PurposeDouble floxed Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoterDepositorUseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-n1-AICD
Plasmid#69925Purposemammalian expression of human AICD fused to EGFPDepositorInsertAICD (APP Human)
UseTagsEGFPExpressionMammalianMutation47 aa longPromoterCMVAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-PAFAH1B1
Plasmid#92430Purposemammalian expression of PAFAH1B1DepositorInsertPAFAH1B1 (PAFAH1B1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine
Plasmid#50464PurposeGi-coupled hM4D-IRES-mCitrine under the control of human synapsin promoteDepositorInserthM4D(Gi)-IRES-mCitrine (CHRM4 Human)
UseAAVTagsHAExpressionMutationPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMK1253
Plasmid#133058PurposeLentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-Clover2 fusion protein.DepositorInsertTau.K18(LM)
UseLentiviralTagsmClover2ExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
CSNK1A1_pLX307
Plasmid#98326PurposeLentiviral expression of CSNK1A1DepositorInsertCSNK1A1 (CSNK1A1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF3197
Plasmid#144673PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSTAT5A (STAT5A Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only