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Plasmid#59947PurposeSplitposon in KanR ColE1 backboneDepositorInsertSplitposon w/ KanR, LacI & PTac
UseTagsExpressionBacterialMutationN/APromoterAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJD427
Plasmid#154371PurposeExpresses FKBP12-XB and SspBcore-FRB from constitutive promotersDepositorInsertproB-RBS(B0032)-SspBcore-FRB-terminator(B0011)-proC-RBS(B0032)-FKBP12-sspBXB-terminator(B0011)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZE31ms2
Plasmid#25860DepositorInsertms2 binding site (x2)
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceNov. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDW115
Plasmid#70062Purposeartificial transcription template "pause ablation"DepositorInsertlambda PR' A+2G T+6G - repeat cassette - E. coli rpoB - lambda TR'
UseTagsExpressionMutationPromoterAvailable sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPTK014-3a-Killer-αMFΔ
Plasmid#84973PurposeType 3a, Killer followed by αMFΔ - secretion signalDepositorInsertKiller followed by αMFΔ secretion tag
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK007-3a-αMFΔ
Plasmid#84966PurposeType 3a, α-mating factor pre-sequence shortened - secretion signalDepositorInsertα-mating factor, pre-sequence shortened secretion tag
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCC48
Plasmid#128285Purposeasymmetric plasmid partitioning E. coli - target plasmidDepositorInsertPiq - mRFP, BBa_J23100 - phlF, parS
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTORGm43GWY
Plasmid#112906PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mCitrine.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTORGm43GWC
Plasmid#112903PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mTFP1.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTORGm43GWF
Plasmid#112904PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mWasabi.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNR186
Plasmid#79552PurposeOutput plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.DepositorInsertGFPmut3b
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPTK008-3a-αMFΔ no Kex
Plasmid#84967PurposeType 3a, α-mating factor no Kex recognition site - secretion signalDepositorInsertα-mating factor no Kex recognition site secretion tag
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbB12c-GFP
Plasmid#126127PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23151Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBzCas13b-gRNA-1
Plasmid#164858PurposeConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.DepositorInsertBzCas13b-gRNA-1
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRETvr-2in1-NN
Plasmid#105126PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneUseTagsmVenus/tagRFPExpressionPlantMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPTK011-3a-SA-αMFΔ
Plasmid#84970PurposeType 3a, Serum albumin followed by αMFΔ - secretion signalDepositorInsertSerum albumin followed by αMFΔ secretion tag
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTEI122
Plasmid#126062PurposeModule 0 vector of PlaMiNGo toolkitDepositorInsertccdB Cassette
UseSynthetic Biology; Module 0TagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0028
Plasmid#162310PurposeLevel 0 C-terminal tag part for MoClo assembly, TTCG-GCTTDepositorInsertC-terminal tag, 9 aa linker, strep tag, stop codon
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB3C5-proB-B0032-E0051
Plasmid#107243PurposeExpresses lacZa-GFP fusion from constitutive proB promoterDepositorInsertProB-RBS(B0032)-Gemini (E0051)
UseTagsExpressionMutationPromoterproBAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB3C5-proC-B0032-E0051
Plasmid#107242PurposeExpresses lacZa-GFP fusion from constitutive proC promoterDepositorInsertProC-RBS(B0032)-Gemini (E0051)
UseTagsExpressionMutationPromoterproCAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only