-
Plasmid#87381PurposeRetroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.DepositorInsertMito-Pericam [ratiometric]
UseRetroviralTagsExpressionMammalianMutationPromoterRetroviral 5' LTR promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
iChr2-Notch Mosaic (SO250)
Plasmid#99752PurposeSecond generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different chromatin localized fluorescent proteins and Notch signalling genesDepositorInsertHIs-H2B-Cherry, H2B-EGFP-V5, HA-H2B-Cerulean, DN-Maml1, NICD-PEST
UseCre/Lox and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (D1399Y)
Plasmid#61358Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; D1399Y mutation i…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV.CAP-B22
Plasmid#175005Purposenon-standard AAV2 rep-AAV.CAP-B22 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation systemDepositorInsertSynthetic construct isolate AAV.CAP-B22 VP1 gene
UseAAVTagsExpressionMammalianMutation7 amino acid substitution between VP1 452 and VP1…Promoterp41Available sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-tdTomato-WPRE
Plasmid#51506PurposeCan be used to generate AAV virus that will express tdTomato in neurons from the synapsin promoterDepositorHas ServiceAAV5InserttdTomato
UseAAVTagsExpressionMutationPromoterphSyn1Available sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-rsChRmine-oScarlet-KV 2.1-WPRE
Plasmid#183523PurposeOptogeneticsDepositorInsertrsChRmine-oScarlet-Kv2.1
UseAAVTagsExpressionMutationI146M/G174SPromoterCaMKIIaAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.LSL.tdTomato
Plasmid#100048PurposeAAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)DepositorInserttdtomato
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-tdTomato-WPRE
Plasmid#51503PurposeCan be used to generate AAV virus that will express tdTomato in the presence of CreDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterpCAGAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.LSL.EGFP
Plasmid#100047PurposeAAV mediated Cre-dependent expression of EGFP (Lox-Stop-Lox)DepositorInsertEGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pAAV-Ef1a DIO-rsChRmine-oScarlet-WPRE
Plasmid#183528PurposeOptogeneticsDepositorInsertrsChRmine-oScarlet
UseAAVTagsExpressionMutationI146M/G174SPromoterEf1aAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
LgBiT-hACE2
Plasmid#173431Purposeprotein expression plasmid of LgBiT-hACE2-IgG1 FcDepositorInsertLgBiT-hACE2-IgG1 Fc (ACE2 Human)
UseLuciferaseTagsIgG1ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
scAAV-Cre
Plasmid#177933PurposeExpresses Cre-recombinase and can be packaged into AAV particles.DepositorInsertCAG-Cre
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Kif2c
Plasmid#208651PurposeMammalian expression plasmid expressing eGFP-Kif2c driven by CMV promoterDepositorInsertKif2c (KIF2C Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
scAAV-sgRNA-GFP
Plasmid#177935PurposeExpresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic miceDepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_DA1m
Plasmid#113049Purposeexpress the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neuronsDepositorUseAAVTagsExpressionMutationPromoterhSynAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_MYC_WT
Plasmid#82927PurposeGateway Donor vector containing MYC, part of the Target Accelerator Plasmid Collection.DepositorInsertMyc (MYC Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a DIO-hsChRmine-oScarlet-WPRE
Plasmid#183526PurposeOptogeneticsDepositorInserthsChRmine-oScarlet
UseAAVTagsExpressionMutationH33RPromoterEf1aAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Δ18 D614G
Plasmid#164437PurposeEncodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids and D614G mutation for pseudovirus productionDepositorInsertSARS-CoV-2 Spike D614G variant truncated to remove 18 amino acids (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_DA1h
Plasmid#113050Purposeexpress the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neuronsDepositorUseAAVTagsExpressionMutationPromoterhSynAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
MEK5 DD(S311D,T315D)-pcw107-V5
Plasmid#64619Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertMAP2K5 (MAP2K5 Human)
UseLentiviralTagsV5ExpressionMutationS311D, T315D*PromoterPGKAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hras (G12V)-pcw107
Plasmid#64603Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertHRAS (HRAS Human)
UseLentiviralTagsExpressionMutationG12VPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-GFAP-NLS-CasRx-NLS-FLAG-U6-DR-SgRNA_Ptbp1-DR
Plasmid#154001PurposeVector expressed CasRx and sgRNA_Ptbp1 for AAV packageDepositorInsertCasRx, Ptbp1 sgRNAs
UseCRISPR and Mouse TargetingTagsFLAGExpressionMutationPromoterGFAP, U6Available sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-GGA3-VHS-GAT
Plasmid#173719PurposeVHS-GAT domain in GGA3 can bind to an active form of ARF1 protein. Using this construct, you can assess the activity of ARF1 protein.DepositorUseTagsGSTExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDHa_mDDX5_FL_wt
Plasmid#88869Purposeconstitutive expression of Ha-DDX5 in mammalian cellsDepositorInsertDDX5 (Ddx5 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
p38 WT O/E (MAPK14)-pcw107-V5
Plasmid#64624Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertMAPK14 (transcript variant 2) (MAPK14 Human)
UseLentiviralTagsV5ExpressionMutationPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
AR-V7-pcw107
Plasmid#64635Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertAR (transcript variant 1, splice isoform) (AR Human)
UseLentiviralTagsExpressionMutationsplice isoform*PromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
AR-V7-pcw107-V5
Plasmid#64636Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertAR (transcript variant 1, splice isoform) (AR Human)
UseLentiviralTagsV5ExpressionMutationsplice isoform *PromoterPGKAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
IKKalpha (S176E,S180E)-pcw107-V5
Plasmid#64608Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertCHUK (CHUK Human)
UseLentiviralTagsV5ExpressionMutationS176E, S180EPromoterPGKAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a DIO-ChRmine-oScarlet-Kv2.1-WPRE
Plasmid#183525PurposeOptogeneticsDepositorInsertChRmine-oScarlet-Kv2.1
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-Dnmt3a3L-sc27
Plasmid#71827PurposeFor bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.DepositorInsertDnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
UseTags6xHis tagExpressionBacterialMutationPromoterAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE
Plasmid#183519PurposeOptogeneticsDepositorInsertChRmine-oScarlet-Kv2.1
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5
Plasmid#64613Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertCtnnb1 (Ctnnb1 Mouse)
UseLentiviralTagsV5ExpressionMutationS33A, S37A, T41A, S45APromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
beta-catenin (S33A, S37A, T41A, S45A)-pcw107
Plasmid#64612Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertCtnnb1 (Ctnnb1 Mouse)
UseLentiviralTagsExpressionMutationS33A, S37A, T41A, S45APromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
beta-catenin (S33Y)-pcw107-V5
Plasmid#64616Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertCTNNB1 (CTNNB1 Human)
UseLentiviralTagsV5ExpressionMutationS33YPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_hygro_3xflag_NRF2(RR)
Plasmid#136535PurposeLentiviral expression vector for an inducible 3xflag-tagged NRF2(a1584c_a1586t_a1589g)DepositorInsertNRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)
UseLentiviral; Destinatioin vector for gateway cloni…Tags3x FLAGExpressionMammalianMutationThis plasmid is developed by mutating 3 base pair…PromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_human_ADAR2_ccC
Plasmid#158112Purposedeletion hADAR2DepositorInsertADAR2 (ADARB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_human_ADAR2_ccB
Plasmid#158113Purposedeletion hADAR2DepositorInsertADAR2 (ADARB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a DIO-ChRmine-oScarlet-WPRE
Plasmid#183524PurposeOptogeneticsDepositorInsertChRmine-oScarlet
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_human_ADAR2_ccA
Plasmid#158114Purposedeletion hADAR2DepositorInsertADAR2 (ADARB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only