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156,554 results

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PB-TRE-dCas9-Zim3-MeCP2

Plasmid

#220143

Purpose

doxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repression

Depositor

Frank Buchholz

Article

Ding et al Life Sci Alliance. 2022 Mar 14;5(6). pii

Insert

dCas9-Zim3-MeCP2

Use

CRISPR and Synthetic Biology; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Tre-3G

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-PIP5K1A

Plasmid

#202720

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:PIP5K1A (PIP5K1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-3XmCherry

Plasmid

#64108

Purpose

Expression of Sp dCas9-3XmCherry in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

3XmCherry

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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Klotho (membrane)

Plasmid

#17712

Depositor

Hal Dietz

Article

Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

Insert

Klotho (KL Human)

Use

Tags

V5

Expression

Mammalian

Mutation

P101S

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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lenti-SpCas9 neo

Plasmid

#104996

Purpose

This lentiviral construct delivers hSpCas9 and G418 resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag

Plasmid

#216250

Purpose

HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

HDR template for human SEC61B (SEC61B HDR template, Human, Synthetic)

Use

CRISPR; Hdr template

Tags

BSD.P2A.miniIAA7.3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pTS26_QUAS_CsChrimson

Plasmid

#175548

Purpose

Expresses the optogenetic channel CsChrimson off of a QUAS promoter and contains Piggybac terminal repeats for transposon-mediated integration

Depositor

Leslie Vosshall

Article

Sorrells et al Elife. 2022 May 12;11:e76663. doi: 10.75

Insert

CsChrimson

Use

Tags

tdTomato

Expression

Insect

Mutation

Promoter

QUAS

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pPROBE-GT

Plasmid

#37814

Depositor

Steven Lindow

Article

Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

Promotorless gfp reporter gene

Use

Tags

Expression

Mutation

Promoter

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only
TFORF0103

Plasmid

#141516

Purpose

Lentiviral vector for overexpressing the LEUTX transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

LEUTX (LEUTX Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-VSVM

Plasmid

#64086

Purpose

Expression vector for Vesicular Stomatitis Virus Indiana strain matrix gene

Depositor

Ian Wickersham

Insert

VSV matrix protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pPM28 (eroGFP CEN/ARS URA3)

Plasmid

#20131

Depositor

Feroz Papa

Article

Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

Insert

eroGFP (KAR2 jellyfish, Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 11, 2009

Availability

Academic Institutions and Nonprofits only

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pPROBE-gfp[LVA]

Plasmid

#40170

Depositor

Steven Lindow

Article

Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

Promotor probe

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pARF6-CFP

Plasmid

#11382

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 6 (ARF6 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-NONO

Plasmid

#127655

Purpose

Over-express HA-tagged human NONO

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

Insert

NONO (NONO Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-Golgi-7

Plasmid

#55052

Purpose

Localization: Golgi Complex, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Golgi (B4GALT1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pSH-EF1a-OsTIR1.F74A-mCherry-IRES-puro

Plasmid

#216247

Purpose

Express OsTIR1.F74A mutant with mCherry tag for AID2

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

OsTIR1.F74A

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Akt-FoxO3a-KTR-EGFP

Plasmid

#125131

Purpose

Fluorescent reporter for Akt activity

Depositor

Kazuhiro Aoki

Article

Maryu et al Cell Struct Funct. 2016 Jul 22;41(2):81-

Insert

FOXO2 (FOXO3 Human)

Use

Tags

Enhanced green fluorescent protein (EGFP)

Expression

Mammalian

Mutation

FoxO3A 1-402 amino acid

Promoter

CAG promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-NL63-Spike

Plasmid

#168929

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-NL63-Spike (HCNV63gp2 )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMX229

Plasmid

#23002

Depositor

Linda Wordeman

Article

Stumpff et al Dev Cell. 2008 Feb . 14(2):252-62.

Insert

Kif18A (KIF18A Human)

Use

Tags

6His and EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro

Plasmid

#99636

Purpose

Empty backbone, 3rd generation lentiviral vector

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Type

Empty backbone

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

Promoter

SFFVp

Available since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221_FLVCR1

Plasmid

#131892

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

FLVCR1 (FLVCR1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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gCH27 (crCD55-4)

Plasmid

#217343

Purpose

crRNA targeting CD55

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

crCD55-4 (CD55 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)IRES GFP

Plasmid

#51406

Purpose

Expresses GFP from internal ribosome entry sequence, cloning component

Depositor

Kathleen_L Collins

Article

Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

Insert

IRES GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pRSII426

Plasmid

#35470

Depositor

Steven Haase

Article

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast 2 micron vector

Tags

Expression

Mutation

Promoter

None

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G

Plasmid

#167937

Purpose

Inducible CRISPRa expression

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

dxCas9(3.7)-VPR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

tetON

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDIRE

Plasmid

#26745

Depositor

Rolf Zeller

Article

Osterwalder et al Nat Methods. 2010 Oct 17

Insert

iCre expression cassette

Use

Cre/Lox; Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pBECKP-km

Plasmid

#117235

Purpose

A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadDE

Plasmid

#193837

Purpose

Express TadDE (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-DualEditor-Cas9D10A-2xUGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-His-hAR-2

Plasmid

#89091

Purpose

baculovirus transfer vector for histidine-tagged full-length human androgen receptor, starts Met-Ala-His6-Met-hAR

Depositor

Elizabeth Wilson

Article

Liao et al Biochemistry. 1999 Jul 27;38(30):9718-27

Insert

hAR (AR Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only
pHLUM (version 2)

Plasmid

#64166

Purpose

harbours marker genes HIS3, LEU2, URA3 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLminP_Luc2P_RE44

Plasmid

#90382

Purpose

Hedgehog - Gli1 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

RUNX

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-6-IPT-9

Plasmid

#197746

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Isopentyl transferase (IPT)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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DHB-mVenus

Plasmid

#136461

Purpose

Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

Depositor

Tobias Meyer, Sabrina Spencer

Article

Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

Insert

DNA Helicase B (HELB Human)

Use

Lentiviral

Tags

mVenus

Expression

Mutation

Promoter

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pmScarlet-HSP70

Plasmid

#163790

Purpose

Expresses mScarlet-Hsp70 in mammalian cells

Depositor

Vincent Timmerman

Article

Adriaenssens et al Sci Rep. 2020 May 29;10(1):8755. doi: 10

Insert

HSP70 (HSPA1A Human)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBTL-2

Plasmid

#22806

Depositor

Ryan Gill

Article

Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

Insert

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3_V5-APEX2-HMOX1

Plasmid

#85824

Purpose

Expressed N-term tagged APEX2 on HMOX1 in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

Insert

V5-APEX2-HMOX1 (HMOX1 Human, Synthetic)

Use

Tags

APEX2 and V5

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCGT-Sos Cat

Plasmid

#32923

Depositor

Dafna Bar-Sagi

Article

Boykevisch et al Curr Biol. 2006 Nov 7;16(21):2173-9.

Insert

SOS1 (SOS1 Human)

Use

Tags

T7 epitope

Expression

Mammalian

Mutation

Contains aa 564-1049 (catalytic domain)

Promoter

CMV

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
pFPV25.1

Plasmid

#20668

Purpose

S. typhimurium rpsM promoter driving gfpmut3 expression in bacteria. Promoter is not regulated by acidic conditions.

Depositor

Raphael Valdivia

Article

Valdivia et al Mol Microbiol. 1996 Oct . 22(2):367-78.

Type

Empty backbone

Use

Tags

GFP mut3

Expression

Bacterial

Mutation

Promoter

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-GFP11-Clathrin light chain

Plasmid

#70217

Purpose

Expresses GFP11-clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP11-Clathrin light chain (CLTA Human)

Use

Tags

GFP11

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
SIRT2 Flag

Plasmid

#13813

Purpose

Mammalian expression of human SIRT2 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT2 (SIRT2 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Transcript variant 2

Promoter

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge

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