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Addgene

pmScarlet-HSP70
(Plasmid #163790)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163790 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pmScarlet_C1
  • Backbone manufacturer
    Addgene (#85042)
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 6606
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HSP70
  • Alt name
    HSPA1A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1926
  • GenBank ID
    NM_005345.6
  • Entrez Gene
    HSPA1A (a.k.a. HEL-S-103, HSP70, HSP70-1, HSP70-1A, HSP70-2, HSP70.1, HSP70.2, HSP70I, HSP72, HSPA1)
  • Promoter CMV
  • Tag / Fusion Protein
    • mScarlet (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV_fwd_primer
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmScarlet-HSP70 was a gift from Vincent Timmerman (Addgene plasmid # 163790 ; http://n2t.net/addgene:163790 ; RRID:Addgene_163790)
  • For your References section:

    BAG3 Pro209 mutants associated with myopathy and neuropathy relocate chaperones of the CASA-complex to aggresomes. Adriaenssens E, Tedesco B, Mediani L, Asselbergh B, Crippa V, Antoniani F, Carra S, Poletti A, Timmerman V. Sci Rep. 2020 May 29;10(1):8755. doi: 10.1038/s41598-020-65664-z. 10.1038/s41598-020-65664-z PubMed 32472079