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PurposeExpresses mScarlet-Hsp70 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 163790 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmScarlet_C1
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Backbone manufacturerAddgene (#85042)
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 6606
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHSP70
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Alt nameHSPA1A
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SpeciesH. sapiens (human)
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Insert Size (bp)1926
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GenBank IDNM_005345.6
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Entrez GeneHSPA1A (a.k.a. HEL-S-103, HSP70, HSP70-1, HSP70-1A, HSP70-2, HSP70.1, HSP70.2, HSP70I, HSP72, HSPA1)
- Promoter CMV
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Tag
/ Fusion Protein
- mScarlet (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer CMV_fwd_primer (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmScarlet-HSP70 was a gift from Vincent Timmerman (Addgene plasmid # 163790 ; http://n2t.net/addgene:163790 ; RRID:Addgene_163790) -
For your References section:
BAG3 Pro209 mutants associated with myopathy and neuropathy relocate chaperones of the CASA-complex to aggresomes. Adriaenssens E, Tedesco B, Mediani L, Asselbergh B, Crippa V, Antoniani F, Carra S, Poletti A, Timmerman V. Sci Rep. 2020 May 29;10(1):8755. doi: 10.1038/s41598-020-65664-z. 10.1038/s41598-020-65664-z PubMed 32472079