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PurposeMammalian expression of human SIRT2 with flag tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13813 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1 +
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSIRT2
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SpeciesH. sapiens (human)
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MutationTranscript variant 2
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Entrez GeneSIRT2 (a.k.a. SIR2, SIR2L, SIR2L2)
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Tag
/ Fusion Protein
- flag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byRoy Frye
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloned into a version of pcDNA3.1 that has been modified to include a C-terminal Flag tag (see author's map for details).
This variant (2) lacks the additional 47 nt present in the 5' end of the coding region in transcript variant 1. Translation is thought to initiate at a downstream in-frame ATG.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SIRT2 Flag was a gift from Eric Verdin (Addgene plasmid # 13813 ; http://n2t.net/addgene:13813 ; RRID:Addgene_13813) -
For your References section:
The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase. North BJ, Marshall BL, Borra MT, Denu JM, Verdin E. Mol Cell. 2003 Feb . 11(2):437-44. 10.1016/S1097-2765(03)00038-8 PubMed 12620231