-
Purpose(Empty Backbone) harbours marker genes HIS3, LEU2, URA3 and MET17
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 64166 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepRS313
-
Modifications to backboneDeleted XhoI and BamHI restriction sites from multiple cloning site by modification of codons, preserving the amino acid sequence of lacZ Introduced an AatII restriction site 5' and XhoI and BamHI sites 3' of HIS3 marker gene Cloned fragment containing LEU2, URA3 and MET17 from pHLUM to XhoI/BamHI RS.
-
Vector typeBacterial Expression, Yeast Expression
-
Selectable markersLEU2, URA3, HIS3 ; MET17
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)XL1 Blue
-
Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHLUM (version 2) was a gift from Markus Ralser (Addgene plasmid # 64166 ; http://n2t.net/addgene:64166 ; RRID:Addgene_64166) -
For your References section:
Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities. Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M. F1000Res. 2016 Sep 20;5:2351. eCollection 2016. 10.12688/f1000research.9606.1 PubMed 27830062