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PPRE-pNL1.3

Plasmid

#84394

Purpose

Secreted Luciferase reporter for PPARgamma activity

Depositor

Severine Degrelle, Thierry Fournier

Article

Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

Insert

PPARG response element (PPARG Human)

Use

Tags

secNluc

Expression

Mammalian

Mutation

Promoter

PPRE

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-neo Fluc EGFP

Plasmid

#90170

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_12GLI-FLuc_R4-R3

Plasmid

#113712

Purpose

Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Insert

12GLI-FLuc

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-neo FlucSM EGFP

Plasmid

#90171

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutation

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Use

Tags

Expression

Mammalian

Mutation

R188Q in FLuc

Promoter

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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Akaluc-N1

Plasmid

#120371

Purpose

Akaluc luciferase mammalian expression in -N1 backbone

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

Akaluc (human optimized)

Use

Tags

Expression

Mammalian

Mutation

Human codon optimized

Promoter

CMV

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc

Plasmid

#117728

Purpose

Integration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc gene

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

NanoLuc

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBEN276

Plasmid

#69150

Purpose

Integration of luciferase operon in Tn7 insertion site of Gram negative bacteria

Depositor

Pierre Germon

Article

Howe et al BMC Microbiol. 2010 Jul 23;10:197. doi:

Insert

luxCDABE

Use

Luciferase

Tags

Expression

Mutation

Promoter

frr

Available since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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p-LCN2

Plasmid

#28225

Depositor

Sebastien Jauliac

Article

Fougere et al Oncogene. 2010 Apr 15. 29(15):2292-301.

Insert

LCN2 promoter (LCN2 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

LCN2

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
pTK305

Plasmid

#66812

Purpose

IVT vector for Fluc mRNA

Depositor

Ron Weiss

Article

Andries et al J Control Release. 2015 Sep 2. pii: S016

Insert

Firefly luciferase

Use

Luciferase; Mrna ivt

Tags

Expression

Mutation

Promoter

T7

Available since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only

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10-4 cyclin E promoter

Plasmid

#8458

Depositor

Bob Weinberg

Article

Geng et al Oncogene 1996 Mar 21;12(6):1173-80.

Insert

human cyclin E promoter (CCNE1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2006

Availability

Academic Institutions and Nonprofits only

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LeGO-Luc2

Plasmid

#154006

Purpose

Lentiviral vector expressing firefly luciferase (Luc2)

Depositor

Boris Fehse

Article

Ellinger et al Viruses. 2020 May 25;12(5). pii: v120505

Insert

Firefly Luciferase

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-VEGFR2-780

Plasmid

#21307

Depositor

Donald Ingber

Article

Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

Insert

VEGFR2 promoter (KDR Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 29, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt2

Plasmid

#86593

Purpose

Expresses eGFP-tagged human Akt2(WT)

Depositor

Ivan Yudushkin, Thomas Leonard

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2 (AKT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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Alpha1

Plasmid

#118044

Purpose

GoldenBraid2.0 Alpha 1 destination vector

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Assembly of gb2.0 transcriptio…

Tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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-1748 human cyclin D1 promoter pGL3Basic

Plasmid

#32726

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase; Reporter gene

Tags

Expression

Mutation

Promoter

-1748CCND1 promoter

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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Omega 1

Plasmid

#118046

Purpose

GoldenBraid2.0 Omega 1 destination vector

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Assembly of gb2.0 transcriptio…

Tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMiniP

Plasmid

#118049

Purpose

GBPart - Minimal Promoter for expression in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

minimal TATA-box promoter with low basal activity

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMTS-hFluc

Plasmid

#177716

Purpose

Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

hFluc

Use

Tags

Mitochondrial Targeting Sequence MTS SS

Expression

Bacterial, Mammalian, and Y…

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTA-Luc SIRT1 promoter (-202)

Plasmid

#10971

Depositor

Toren Finkel

Article

Nemoto et al Science. 2004 Dec 17. 306(5704):2105-8.

Insert

SIRT1 promoter (Sirt1 Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pGL3-VEGFR2-225

Plasmid

#21306

Depositor

Donald Ingber

Article

Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

Insert

VEGFR2 promoter (KDR Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 29, 2009

Availability

Academic Institutions and Nonprofits only