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Addgene

pCI-neo FlucSM EGFP
(Plasmid #90171)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90171 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCIneo
  • Backbone manufacturer
    promega
  • Backbone size w/o insert (bp) 5472
  • Total vector size (bp) 7860
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FLuc EGFP
  • Species
    firefly
  • Insert Size (bp)
    2373
  • Mutation
    R188Q in FLuc

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-neo FlucSM EGFP was a gift from Franz-Ulrich Hartl (Addgene plasmid # 90171 ; http://n2t.net/addgene:90171 ; RRID:Addgene_90171)
  • For your References section:

    Firefly luciferase mutants as sensors of proteome stress. Gupta R, Kasturi P, Bracher A, Loew C, Zheng M, Villella A, Garza D, Hartl FU, Raychaudhuri S. Nat Methods. 2011 Sep 4;8(10):879-84. doi: 10.1038/nmeth.1697. 10.1038/nmeth.1697 PubMed 21892152