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LentiCas9-sgCh2-2-Blast

Plasmid

#199645

Purpose

Expresses Cas9 and sgRNA control guide targeting intergenic region

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsMago_I

Plasmid

#146579

Purpose

Mammalian Expression of HsMago

Depositor

Elisa Izaurralde

Article

Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

Insert

HsMago (MAGOH Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsMago_I

Plasmid

#146582

Purpose

Mammalian Expression of HsMago

Depositor

Elisa Izaurralde

Article

Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

Insert

HsMago (MAGOH Human)

Use

Tags

Expression

Mammalian

Mutation

one non silent mutation Y123C compared to the seq…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CD40LG-COMP5AP-AviTag-9xHis

Plasmid

#157200

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CD40LG (CD40LG Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6

Plasmid

#192789

Purpose

aGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

antiGFP nanobody minimizer

Use

Tags

H6 and ybbR

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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Opto-GPR4

Plasmid

#106001

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR4; Opto-GPR4; A3)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR4 (GPR4 Bovine, Human)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6s

Plasmid

#40753

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

GENIE Project, Douglas Kim

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Insert

GCaMP6s

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pTECH-chAcK3RS(IPYE)

Plasmid

#104069

Purpose

Expression plasmid encoding chAcK3RS(IPYE) and pylT

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Plpp chAcK3RS(IPYE); PproK pyl

Use

Tags

Expression

Bacterial

Mutation

Contains activating mutations V31I, T56P, H62Y, A…

Promoter

Plpp and PproK

Available since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCBL101-RUBY

Plasmid

#199723

Purpose

Expresses betalain biosynthesis genes under CaMV 35S promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

35S-RUBY

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pUC18R6KT-mini-Tn7T-Km

Plasmid

#64969

Purpose

mini-Tn7 base vector with transcriptional terminator and oriT

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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