Skip to main content
Addgene
Showing: 561 - 600 of 1445 results

  1. pSems-leader-mEGFP-MBP-TMD(ALA7)

    Plasmid
    #193051
    Purpose
    Expression of artificial transmembrane protein tagged with mEGFP and MBP at the plasma membrane. Control construct for single molecule co-localization and co-tracking microscopy.
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    ALA7-TMD
    Use
    Tags
    Ig k-chain leader sequence, MBP, and mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    193051-plasmid-map-sequence-id-386362

    Enlarge

  2. pMG051 MBP-β-catenin-His, GST-CK1α

    Plasmid
    #154072
    Purpose
    Co-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.
    Inserts
    CTNNB1 (CTNNB1 Human)
    CSNK1A1 (CSNK1A1 )
    Use
    Tags
    GST, His6, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Tac
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    154072-plasmid-map-sequence-id-308806

    Enlarge

  3. pHR myc-MBP-SspB-iRFP-NLS

    Plasmid
    #223286
    Purpose
    An SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system.
    Depositor
    Jared Toettcher
    Article
    Brumbaugh-Reed et al bioRxiv 2024.01.16.575860
    Insert
    Myc-MBP-SspB-irFP-NLS
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    223286-plasmid-map-sequence-id-442283

    Enlarge

  7. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

    Plasmid
    #53225
    Purpose
    human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis
    Depositor
    Jesse Rinehart
    Insert
    MEK1 (MAP2K1 Human)
    Use
    Phosphoprotein synthesis
    Tags
    HIS and MBP
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli, both codons for Ser21…
    Promoter
    pLtetO
    Available since
    May 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    53225-plasmid-map-sequence-id-82901

    Enlarge

  18. pOCC120-MBP-DDX3X(D354V)-monoGFP-His

    Plasmid
    #219986
    Purpose
    DDX3X(D354V) protein purification
    Depositor
    Simon Alberti
    Insert
    DDX3X(D354V) (DDX3X Human)
    Use
    Tags
    MBP and monoGFP and 6xHis
    Expression
    Insect
    Mutation
    D354V
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219986-plasmid-map-sequence-id-436838

    Enlarge

  19. pOCC120-MBP-DDX3X(G325E)-monoGFP-His

    Plasmid
    #219987
    Purpose
    DDX3X(G325E) protein purification
    Depositor
    Simon Alberti
    Insert
    DDX3X(G325E) (DDX3X Human)
    Use
    Tags
    MBP and monoGFP and 6xHis
    Expression
    Insect
    Mutation
    G325E
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219987-plasmid-map-sequence-id-436837

    Enlarge

Showing: 561 - 600 of 1445 results