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The Histone Chaperone FACT Induces Cas9 Multi-turnover Behavior and Modifies Genome Manipulation in Human Cells.

Wang AS, Chen LC, Wu RA, Hao Y, McSwiggen DT, Heckert AB, Richardson CD, Gowen BG, Kazane KR, Vu JT, Wyman SK, Shin JJ, Darzacq X, Walter JC, Corn JE
Mol Cell. 2020 Jul 16;79(2):221-233.e5. doi: 10.1016/j.molcel.2020.06.014. Epub 2020 Jun 29. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
159993T7pr-His6-MBP-TEV-Cas9-BirA*Bacterial expression of Cas9-BirA*
159994T7pr-His6-MBP-TEV-dCas9-BirA*Bacterial expression of dCas9-BirA*

Antibodies from Article