T7pr-His6-MBP-TEV-Cas9-BirA*
(Plasmid
#159993)
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PurposeBacterial expression of Cas9-BirA*
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159993 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepML-2CT
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Backbone manufacturerMacroLab, UC Berkeley
- Backbone size w/o insert (bp) 4766
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9-BirA*
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SpeciesStreptococcus pyogenes
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Insert Size (bp)6492
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MutationD10A, H840A
- Promoter T7
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Tag
/ Fusion Protein
- 6X-His, MBP, 3X-FLAG, NLS (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 Promoter
- 3′ sequencing primer T7 Terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
T7pr-His6-MBP-TEV-Cas9-BirA* was a gift from Jacob Corn (Addgene plasmid # 159993 ; http://n2t.net/addgene:159993 ; RRID:Addgene_159993) -
For your References section:
The Histone Chaperone FACT Induces Cas9 Multi-turnover Behavior and Modifies Genome Manipulation in Human Cells. Wang AS, Chen LC, Wu RA, Hao Y, McSwiggen DT, Heckert AB, Richardson CD, Gowen BG, Kazane KR, Vu JT, Wyman SK, Shin JJ, Darzacq X, Walter JC, Corn JE. Mol Cell. 2020 Jul 16;79(2):221-233.e5. doi: 10.1016/j.molcel.2020.06.014. Epub 2020 Jun 29. 10.1016/j.molcel.2020.06.014 PubMed 32603710