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Plasmid#80922PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only
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pCMV_UdgX-APOBEC1-UdgX-NG-nCas9
Plasmid#163562PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-APOBEC1-UdgX-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…PromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFL-SV40
Plasmid#115352Purposefirefly luciferase (Fluc) reporter gene driven by SV40 promoterDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1 mCherry-NLS
Plasmid#58476PurposeExpresses a nuclear-targeted mCherry control construct, on a CMV reporterDepositorInsert3X NLS with 3X stop codons
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pALPS gag
Plasmid#101324PurposeEncodes codon optimized NL4-3 gagDepositorInsertNL4-3 gag
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMVmin-luc2
Plasmid#194384PurposeReporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoterDepositorInsert6xCRE-CMVmin-luc2
UseTagsExpressionMammalianMutationPromoterCMV minimalAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
CKAR
Plasmid#14860DepositorInsertC kinase activity reporter
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-28GG
Plasmid#104655PurposeLevel 2 cloning vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8069 - ACTB GFP-P2A + RAB11A mCherry-P2A CLIP Donor
Plasmid#202689PurposeCLIP donor template to generate 2 knock-ins: GFP into ACTB, and mCherry in RAB11A.DepositorInsertsCopGFP
mCherry
UseTagsExpressionMutationPromoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRlsinPGK_H2BGFP_WPRE
Plasmid#91788PurposeConstitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.DepositorInsertHuman histone H2B (H2BC11 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterPGKAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
miniCMV-(mNeonGreen)4-tDeg
Plasmid#129402Purpose(mNeonGreen)4-tDeg fluorogenic proteinDepositorInsert(mNeonGreen)-tDeg
UseTagsExpressionMammalianMutationPromoterminiCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7
Plasmid#11795Purpose3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromotermouse U6Available sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Glt1-eGFP
Plasmid#176862PurposeExpresses Glt-1 fused with GFP at the C-terminus in astrocytesDepositorInsertGlt1-eGFP
UseAAVTagsGFPExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMVTNT-T7-KPNA4
Plasmid#26680DepositorInsertKaryopherin Alpha 4 (KPNA4 Human)
UseTagsT7ExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cys-ELP(120nm)-LPETGG
Plasmid#90472PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGGDepositorInsertCys-ELP(120nm)-LPETGG
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-TFAP4
Plasmid#110156PurposeExpression of human TFAP4 in mammalian cellsDepositorInsertTFAP4 (transcription factor AP-4) (TFAP4 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-N1-STAT3
Plasmid#111934PurposeExpresses human STAT3 fused to GFP in mammalian cellsDepositorInsertSignal transducer and activator of transcription 3 (STAT3 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Recall-miniCMV-sfGFP
Plasmid#137995PurposeBackbone for generation of ClonMapper Recall PlasmidDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
TB:5xNF- κβ_RE:MiniP
Plasmid#133878Purpose5 copies of the NF-κβ DNA binding motifDepositorInsert5 copies of the NF-kb DNA binding motif
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-FAB(ER-LD)
Plasmid#214420PurposeDetection of ER-lipid droplet contact siteDepositorInsertRspAN-FRB-V5-ER_IRES_CFAST-20nm-Halo-6xHp
UseLentiviralTagsCFAST-Halo and RspAN-FRB-V5ExpressionMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-tdTomato
Plasmid#159275PurposeHuman AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.DepositorInserttdTomato
UseAAV; S1 knockin donor vectorTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSU250
Plasmid#173837PurposeExpresses 250 kD Penn State ladder proteinDepositorInsertSTRHSTPABPACIL1bQRSSTRHSTPABMBPpepN
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pFBOT563
Plasmid#40864DepositorInsertOxytocin promoter
UseTagsExpressionBacterial and InsectMutationPromotermouse oxytocin promoterAvailable sinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Golgi-7
Plasmid#55052PurposeLocalization: Golgi Complex, Excitation: 587, Emission: 610DepositorInsertGolgi (B4GALT1 Human)
UseTagsmCherryExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLX311-Cas9
Plasmid#118018PurposeConstitutive expression of Cas9DepositorInsertCas9
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-EF1a-D614G-N501Y
Plasmid#177939PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.DepositorInsertSARS-CoV-2 Spike protein (D614G, N501Y) (S )
UseTagsExpressionMammalianMutationD614G, N501YPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
RAD21 C-tag CRISPR
Plasmid#140540PurposeRAD21 tagging CRISPRDepositorInsertRAD21 (RAD21 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-flex-MCS--4x6T
Plasmid#196420PurposeEmpty vector for astrocyte-selective Cre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassetteDepositorTypeEmpty backboneUseAAV and Cre/Lox; Astrocyte-selectiveTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9
Plasmid#163561PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-APOBEC1-UdgX-HF-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…PromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta1(617-949)-GFP
Plasmid#100732PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP1B1 (AP1B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)
Plasmid#62956PurposeSinglet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.DepositorInsertT2A-Gal4-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2_ubi:EGFP
Plasmid#27323DepositorInsertubiquitin:EGFP reporter
UseTol2 transposonTagsExpressionMutationPromoterAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP(S65T)-His3MX6
Plasmid#41598DepositorInsertHis3
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionYeastMutationPromoterTEFAvailable sinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-C12orf66
Plasmid#87047Purposemammalian expression of C12orf66DepositorInsertC12orf66 (C12orf66 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBMN mEGFP-NDP52(V136S)
Plasmid#119682PurposeFor mammalian expression of LIR mutant NDP52DepositorInsertNDP52 (CALCOCO2 Human)
UseRetroviralTagsmEGFPExpressionMammalianMutationV136SPromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGG-B1
Plasmid#78504PurposeTrans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsidDepositorInsertsAAV2 Rep
AAV-B1 capsid
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSP573
Plasmid#98796PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFQS-TEV WT
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-BRD4
Plasmid#204692PurposeThis plasmid allows for inducible expression of short BRD4 isoform tagged with mNeonGreen, in mammalian cells.DepositorInsertBRD4 (BRD4 Human)
UseTagsmNeonGreenExpressionMammalianMutationn/aPromoterCMVAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-D156R (ttLbCas12a)
Plasmid#195366PurposeA temperature-tolerant LbCas12a mutant (D156R), also known as ttLbCas12aDepositorInsertLbCas12a-D156R (ttLbCas12a)
UseCRISPR; Gateway compatible lbcas12a-d156r (ttlbca…TagsExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF-L3-NF-L1-dMb2Cas12a
Plasmid#177686PurposeGateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editingDepositorInsert2xFokI-dMb2Cas12a
UseCRISPR; Gateway compatible 2xfoki-dmb2cas12a entr…TagsNLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNTI827
Plasmid#216635PurposeCal-ID-ERM (V5) ER membrane-localized Cal-ID, mammalian expression and lentivirus productionDepositorArticleInsertCal-ID-ERM
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyVec5
Plasmid#34792DepositorTypeEmpty backboneUseHydra expressionTagsActin/RFP fusion proteinExpressionMutationPromoterHydra vulgaris actin promoterAvailable sinceJan. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMIEG3-c-Jun
Plasmid#40348DepositorInsertc-Jun (JUN Human)
UseRetroviralTagsIRES eGFPExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-ITFG2
Plasmid#87044Purposemammalian expression of ITFG2DepositorInsertITFG2 (ITFG2 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLSSmOrange-mKate2 caspase-3 biosensor
Plasmid#37132DepositorInsertLSSmOrange-DEVD-mKate2
UseTagsExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-flag S127A YAP
Plasmid#27370DepositorInsertYAP (YAP1 Human)
UseTagsflagExpressionMammalianMutationS127A ( major phosphorylation site in YAP2)PromoterAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENTRY-GFP
Plasmid#15301DepositorInserthrGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
ER_K0
Plasmid#133863PurposeExpression of ER-targeted Ribosome non-stalling reporterDepositorInsertER_K0
UseTagsEGFP and RFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only