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Stable expression of large transgenes via the knock-in of an integrase-deficient lentivirus.

Chavez M, Rane DA, Chen X, Qi LS
Nat Biomed Eng. 2023 May 1. doi: 10.1038/s41551-023-01037-x. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
202687pSLQ8001 - pCMV-R8.91 (D64V)R8.91 plasmid with mutated integrase (D64V)
202688pSLQ9663 - ACTB GFP-P2A CLIP DonorCLIP donor template for integrating GFP into ACTB locus. Encodes GFP with ACTB homology arms.
202689pSLQ8069 - ACTB GFP-P2A + RAB11A mCherry-P2A CLIP DonorCLIP donor template to generate 2 knock-ins: GFP into ACTB, and mCherry in RAB11A.
202690pSLQ8079 - IL2RG GFP-P2A CLIP DonorCLIP donor template for integrating GFP into IL2RG locus.
210015pSLQ6332 - ACTB CRISPRa CLIP DonorCLIP donor for integrating CRISPRa (dCas12-miniVPR)-T2A-GFP-P2A to the ACTB locus

Antibodies from Article