pSLQ8001 - pCMV-R8.91 (D64V)
(Plasmid
#202687)
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PurposeR8.91 plasmid with mutated integrase (D64V)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 202687 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Growth instructionsRecombinations are commonly observed for this plasmid. Recombination deficient strains recommended.
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Copy numberUnknown
Gene/Insert
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Gene/Insert namegag pol
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MutationD64V
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene Note: This is a lentiviral packaging plasmid that may contain regulatory and accessory HIV genes. Please see the associated article and full sequence for more details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSLQ8001 - pCMV-R8.91 (D64V) was a gift from Stanley Qi (Addgene plasmid # 202687 ; http://n2t.net/addgene:202687 ; RRID:Addgene_202687) -
For your References section:
Stable expression of large transgenes via the knock-in of an integrase-deficient lentivirus. Chavez M, Rane DA, Chen X, Qi LS. Nat Biomed Eng. 2023 May 1. doi: 10.1038/s41551-023-01037-x. 10.1038/s41551-023-01037-x PubMed 37127707