pSLQ6332 - ACTB CRISPRa CLIP Donor
(Plasmid
#210015)
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PurposeCLIP donor for integrating CRISPRa (dCas12-miniVPR)-T2A-GFP-P2A to the ACTB locus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 210015 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
- Backbone size w/o insert (bp) 8000
- Total vector size (bp) 14138
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedLbCpf1-miniVPR-T2A-GFP
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Insert Size (bp)5463
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSLQ6332 - ACTB CRISPRa CLIP Donor was a gift from Stanley Qi (Addgene plasmid # 210015 ; http://n2t.net/addgene:210015 ; RRID:Addgene_210015) -
For your References section:
Stable expression of large transgenes via the knock-in of an integrase-deficient lentivirus. Chavez M, Rane DA, Chen X, Qi LS. Nat Biomed Eng. 2023 May 1. doi: 10.1038/s41551-023-01037-x. 10.1038/s41551-023-01037-x PubMed 37127707