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Molecular features driving condensate formation and gene expression by the BRD4-NUT fusion oncoprotein are overlapping but distinct.
Kosno M, Currie SL, Kumar A, Xing C, Rosen MK
Sci Rep. 2023 Jul 24;13(1):11907. doi: 10.1038/s41598-023-39102-9.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
204692pcDNA5-FRT/TO-mNeonGreen-BRD4This plasmid allows for inducible expression of short BRD4 isoform tagged with mNeonGreen, in mammalian cells.
204693pcDNA5-FRT/TO-mNeonGreen-NUTThis plasmid allows for inducible expression of full-length NUT tagged with mNeonGreen, in mammalian cells.
204694pcDNA5-FRT/TO-mNeonGreen-BRD4-NUTThis plasmid allows for inducible expression of full-length BRD4-NUT fusion tagged with mNeonGreen, in mammalian cells.
204695pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(MIN)This plasmid allows for inducible expression of BRD4-NUT(MIN) mutant fusion tagged with mNeonGreen, in mammalian cells.
204696pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(deltaMIN)This plasmid allows for inducible expression of BRD4-NUT(deltaMIN) mutant fusion tagged with mNeonGreen, in mammalian cells.
204697pcDNA5-FRT/TO-mNeonGreen-BRD4-p300This plasmid allows for inducible expression of engineered BRD4-p300 fusion tagged with mNeonGreen, in mammalian cells.
204698pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(H*IT)This plasmid allows for inducible expression of engineered BRD4-p300(H*IT) fusion tagged with mNeonGreen, in mammalian cells.
204699pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(HI)This plasmid allows for inducible expression of engineered BRD4-p300(HI) fusion tagged with mNeonGreen, in mammalian cells.
204700pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(I)This plasmid allows for inducible expression of engineered BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells.
204701pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(deltaI)This plasmid allows for inducible expression of engineered BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells.

Antibodies from Article