-
Plasmid#138504PurposeA-to-G base editorDepositorInsertecTadA(8e)-dLbCas12a
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBT44c
Plasmid#138511PurposeP2DepositorInsertinteinC-dSpCas9-UGI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
ND4.2-Left TALE-G1397-N-DddA11
Plasmid#179681PurposeExpression of base editor in mammalian cellsDepositorInsertCOX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_ATP5B 3'UTR
UseTagsExpressionMammalianMutationS1330I A1341V N1342S E1370K T1380IPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
PE_CO
Plasmid#190335PurposePrime editor (PE2) with codon-optimized Reverse transcriptase for prime editing in mammalian cellsDepositorInsertPE2 with codon-optimized reverse transcriptase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Marathon-RT pentamutant for Split-PE (pEH107)
Plasmid#190110PurposeExpresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)DepositorInsertMarathon-RT
UseTagsbpNLSExpressionMammalianMutationD14R, N26R, D74R, N116K, N197RPromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-NLRC5
Plasmid#37521DepositorInsertNLRC5 (NLRC5 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Matr3 delRRM2
Plasmid#32882DepositorInsertMATR3 Matrin3 (MATR3 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationdeleted amino acids 497-566PromoterCMVAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Matr3 delRRM1
Plasmid#32881DepositorInsertMATR3 Matrin3 (MATR3 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationdeleted amino acids 399-468PromoterCMVAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-α-synuclein
Plasmid#205713PurposeMammalian expression of mNeonGreen-alpha-synucleinDepositorInsertalpha-synuclein (SNCA Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-surrogate-reporter
Plasmid#65624PurposeFluorescent reporter to monitor the activty of Bxb1DepositorInsertattP
UseBxb1TagsExpressionMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
xCas9 3.6
Plasmid#108384PurposeMammalian expression vector for xCas9 3.6DepositorInsertxCas9 3.6
UseTagsNLSExpressionMammalianMutationE108G, S217A, A262T, S409I, E480K, E543D, M694I, …PromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
Calnexin-HT-mGold
Plasmid#183987PurposeExpresses fluorescent HaloTag7 fusion protein in mammalian cells with localization in the ER.DepositorInsertCalnexin (CANX Human)
UseTagsHaloTag, mGoldExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
DD-DamV133A-tdTomato-LMNB1
Plasmid#159604PurposeShield-1 inducible transient mammalian expression of Dam-tdTomato-LMNB1 for DamID, containing the V133A mutant of Dam and a fused tdTomato to monitor fusion protein localization and expression levels.DepositorInsertDD-DamV133A-tdTomato-LMNB1
UseTagsExpressionMammalianMutationDam V133A mutationPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Marathon-RT tetramutant for Split-PE (pEH101)
Plasmid#190109PurposeExpresses Marathon-RT tetramutant (D14R-D74R-N116K-N197R)DepositorInsertMarathon-RT
UseTagsbpNLSExpressionMammalianMutationD14R, D74R, N116K, N197RPromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
Ensconsin-18-mEos2
Plasmid#99230PurposeMammalian expression of the photoconvertible protein mEos2 as an Ensconsin fusion.DepositorInsertmEos2
UseTagsEnsconsin (MAP7)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
Calnexin-HT8-mGold
Plasmid#183989PurposeExpresses fluorescent HaloTag8 fusion protein in mammalian cells with localization in the ER.DepositorInsertCalnexin (CANX Human)
UseTagsHaloTag8 (C61S, C262S), mGoldExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
MMLV-RT for Split-PE (pEH45)
Plasmid#190105PurposeExpresses MMLV-RT (full-length, from PE2) for Split-PE useDepositorInsertMMLV-RT
UseTagsbpNLSExpressionMammalianMutationRT pentamutant from PE2 (D200N, T306K, W313F, T33…PromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
AMPK-β2-MYC
Plasmid#40606DepositorUseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
TPI-PTC1
Plasmid#130699PurposeExpresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid)DepositorInsertTPI-PTC1 (TPI1 Human)
UseTagsExpressionMammalianMutationPTC1PromoterPcmvAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only