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Addgene
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  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    .... Find pSpCas9(BB)-2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited by the Bob Weinberg lab.... It can be used with packaging plasmids such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral..., this plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral...

  3. Pooled Library Amplifications

    Type
    Blog Post
    ... Protoc. 2017 with modifications Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled...1866 bp, plus a 20 bp barcode 400 ng Solid pCMV-Rep 78/68 SSM Library Amplification and Sequencing...

  5. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...158008 pCMV-mGold-Tubulin-C-18 Microtubules alpha-tubulin mGold Francois St-Pierre 158009 pCMV-mGold-Actin-C...158004 pCMV-mGold-Calnexin-N-14 Endoplasmic Reticulum Calnexin mGold Francois St-Pierre 158002 pCMV-mGold-Rab4a-C...GFP Mitochondria ABCb10 GFP Orian Shirihai 158003 pCMV-mGold-CAF1-C-10 Nucleus CAF-1 mGold Francois St-...Mitochondria-Outer Membrane Mff acGFP1 Gia Voeltz 158007 pCMV-mGold-Mito-N-7 Mitochondria Cox8A mGold Francois...anchor transmembrane domain oxBFP Erik Snapp 158005 pCMV-mGold-MannII-N-10 Golgi apparatus MannII mGold Francois... Late endosomes RAB7 DsRed Richard Pagano 158006 pCMV-mGold-Lysosome-N-20 Lysosome Lamp1 mGold Francois...

  6. Cre-lox system

    Type
    Collection
    ...Expression System PI 8394 p209 pCMV-cre-K Cre-K CMV Mammalian Green 8395 p210 pCMV-CREM CREM CMV Mammalian Green...Lentiviral Trono 12493 p259 pCMV-CRE-M (BglII) CREM CMV Mammalian Green 12494 p260 pCMV-CRE-M-AR(wt) CREM fused...Simpson 106368 pCMV-Tag2B-NCre N-terminal split-Cre CMV Mammalian Hirrlinger 106369 pCMV-Tag3B-CCre C-terminal...Hirrlinger 106370 pCMV-Tag2B-NCreERT2 N-terminal split-Cre-ERT2 CMV Mammalian Hirrlinger 106371 pCMV-Tag3B-ERT2CCre... Green 8401 p224 pCMV-RFP/CREM floxed RFP within CREM CMV Mammalian Green 8403 p153 pPr-cre Cre probasin...ligand binding domain CMV Mammalian Green 12496 p270 pCMV-CRE-M-AR(LNCaP) CREM fused to mutant AR ligand binding... binding domain CMV Mammalian Green 12497 p274 pCMV-CRE-M-AR(T) CREM fused to mutant AR ligand binding...

  8. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...be used with packaging plasmid pCMV-dR8.2 dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg’s lab...Note: pLKO.1 could also be packaged using pCMV-dR8.2 dvpr and pCMV-VSVG from the Robert Weinberg lab. For...

  9. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...below: ID Plasmid Description 47948 3xFLAG-dCas9/pCMV-7.1 enChIP plasmid, expresses 3xFLAG-dCas9 in mammalian...iChIP plasmid, LexA-binding elements 48874 3xFNLDD/pCMV-7.1 iChIP plasmid, expresses 3xFNLDD in mammalian...specific genomic regions of interest 63589 3xFN-Tel-TAL/pCMV-7.1 Expresses a 3xFLAG-tagged TAL protein recognizing...

  10. Lentivirus Plasmids

    Type
    Collection
    ...plasmid Trono 8454 pCMV-VSV-G N/A Envelope VSV-G-expressing envelope plasmid Weinberg 8455 pCMV-dR8.2 dvpr 2nd...and 3rd generation transfer plasmids Trono 12263 pCMV delta R8.2 2nd Packaging Contains Gag, Pol, Rev,...

  11. Genetic Code Expansion

    Type
    Collection
    ...164080 pCMV-MtPylRS (human-opti) PylRS M. thermophila acetyl-lysine Mammalian Tao Liu 164081 pCMV-MfPylRS...164195 pCMV-MtAcKRS (human-opti) AcKRS M. thermophila acetyl-lysine Mammalian Tao Liu 164196 pCMV-MfBulKRS...SepRS9 E. coli Sep Bacterial TAG Jesse Rinehart 71403 pCMV-DnpK PylRS M. barkeri N6‐(2‐(2,4‐dinitrophenyl)acetyl..., DiZSeK, or DiZHSeC Bacterial TAG P. Chen 91706 pCMV-MbPylRS(DiZPK) PylRS M. barkeri photocrosslinkers...azidonorleucine (Anl) Mammalian Elizabeth Kirby 223508 pCMV_NESPylRS(AF)_hU6tRNAPyl PylRS (Y306A, Y384F) M. mazei...mazei Mammalian TAG Edward Lemke 223509 pCMV_NESPylRS(WT)_hU6tRNAPyl PylRS M. mazei Mammalian TAG Edward ...

  13. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly...

  16. Retrovirus Plasmids

    Type
    Collection
    ...pBS-CMV-gagpol Packaging MoMLV gag and pol Salmon 8454 pCMV-VSV-G Envelope Envelope protein; use with packaging...

  17. Luciferase Plasmids

    Type
    Collection
    ...target sequence in the 3' UTR David Bartel 101139 pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase...of Venus-firefly luciferase Roland Friedel 170575 pCMV-FLuc Firefly CMV Retroviral expression of firefly...

  18. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... activity; protein purification vector  87439  pCMV-HF-BE3  HF-Cas9 (NGG)  Very low off-target activity...

  19. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Ding 186012 pCMV Flag-LRRK2 K17A/K18A LRRK2 Flag CMV Parkinson's Suzanne Pfeffer 186013 pCMV Flag-LRRK2...Cheryl Arrowsmith 213544 pCMV-mKO2-Parkin PRKN mKO2 CMV Parkinson's Lani Wu 213545 pCMV-Parkin(C431N)-A92mKO2...Parkinson's Lani Wu 213546 pCMV-Parkin(E16A) PRKN CMV Parkinson's Lani Wu 213547 pCMV-Parkin PRKN CMV Parkinson's...Spinocerebellar ataxia, infantile onset Smita Patel 205706 pCMV-alphaS-2 SNCA CMV Parkinson's Randy Schekman 205713...Parkinson's Lani Wu 213548 pCMV-Parkin-A92mKO2 PRKN mKO2 CMV Parkinson's Lani Wu 213549 pLv-CMV(trunc)-Parkin-A92mKO2...Dong-Er Zhang 13313 pCMVTNT PINK1 N-myc PINK1 Myc CMV Parkinson's Mark Cookson 13314 pCMVTNT PINK1 C-myc PINK1...Jens Schwamborn 87085 pCMV4-ApoE2 APOE CMV Alzheimer's Bradley Hyman 87086 pCMV4-ApoE3 APOE CMV Alzheimer's...
Showing: 1 - 20 of 20 results