Calnexin-HT-mGold
(Plasmid
#183987)
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PurposeExpresses fluorescent HaloTag7 fusion protein in mammalian cells with localization in the ER.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183987 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCalnexin
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SpeciesH. sapiens (human)
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Insert Size (bp)3438
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Entrez GeneCANX (a.k.a. CNX, IP90, P90)
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Tag
/ Fusion Protein
- HaloTag, mGold (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Calnexin-HT-mGold was a gift from Pablo Rivera-Fuentes (Addgene plasmid # 183987 ; http://n2t.net/addgene:183987 ; RRID:Addgene_183987) -
For your References section:
A locally activatable sensor for robust quantification of organellar glutathione. Emmert S, Quargnali G, Thallmair S, Rivera-Fuentes P. Nat Chem. 2023 Oct;15(10):1415-1421. doi: 10.1038/s41557-023-01249-3. Epub 2023 Jun 15. 10.1038/s41557-023-01249-3 PubMed 37322101