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pUCmini-iCAP-AAV.CAP-Mac

Plasmid

#200658

Purpose

Synthetic construct isolate AAV.CAP-Mac VP1 gene

Depositor

Viviana Gradinaru

Article

Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

Insert

Synthetic construct isolate AAV.CAP-Mac VP1 gene

Use

AAV

Tags

Expression

Mammalian

Mutation

7 amino acid insertion after Q588 of the AAV9 CAP…

Promoter

p41

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-JEDI-2P-Kv-WPRE

Plasmid

#179463

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Has Service

AAV9

Insert

JEDI-2P-Kv

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEx-iSeroSnFR

Plasmid

#128486

Purpose

Fluorescent reporter for serotonin (conditional + membrane localization tag)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Has Service

AAV9

Insert

iSeroSnFR

Use

AAV

Tags

Ig-kappa leader, Myc, PDGFR, and cpsfGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SomaGCaMP6f2

Plasmid

#158757

Purpose

Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker, and the EE-RR coiled coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Has Service

AAV8 and AAV9

Insert

SomaGCaMP6f2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pIRES2-hPAC

Plasmid

#129488

Purpose

Expresses human TMEM206

Depositor

Zhaozhu Qiu

Article

Yang et al Science. 2019 Apr 26;364(6438):395-399.

Insert

Transmembrane protein 206 (PACC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Zebrafish PAC (fPAC)

Plasmid

#129498

Purpose

Expresses zebrafish TMEM206. In pcDNA3.1(+) backbone

Depositor

Zhaozhu Qiu

Article

Yang et al Science. 2019 Apr 26;364(6438):395-399.

Insert

zebrafish PAC (pacc1 Zebrafish)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pIRES2-hPAC-FLAG

Plasmid

#129494

Purpose

Expresses human TMEM206 tagged at the C-terminus with FLAG

Depositor

Zhaozhu Qiu

Article

Yang et al Science. 2019 Apr 26;364(6438):395-399.

Insert

human PAC tagged with FLAG tag at the C-terminal end (PACC1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-dTom-nlsdTom

Plasmid

#135630

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneurons under the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, and AAV9

Insert

dTom-nlsdTom

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-Gq-P2A-dTomato

Plasmid

#135635

Purpose

AAV vector to drive the expression of Gq-DREADD-P2A-dTomato in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, and AAV9

Insert

Gq-P2A-dTomato

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-GFP-fGFP

Plasmid

#135631

Purpose

AAV vector to drive the expression of fGFP in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, and AAV9

Insert

GFP-fGFP

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-GCaMP6f

Plasmid

#135632

Purpose

AAV vector to drive the expression of GCaMP6f in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, and AAV9

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-ChR2-mCherry

Plasmid

#135634

Purpose

AAV vector to drive the expression of ChR2-mCherry in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, and AAV9

Insert

ChR2-mCherry

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-C1V1-eYFP

Plasmid

#135633

Purpose

AAV vector to drive the expression of C1V1-eYFP in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, AAV5, and AAV9

Insert

C1V1-eYFP

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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