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Plasmid#15300Purposea Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORFDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only
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pBadcrtEBI_13
Plasmid#63811Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5E-EF1a/b-actin
Plasmid#82583Purpose5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expressionDepositorInsertEF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-FAK W266A
Plasmid#186149PurposeFor expressing FAK in mammalian cellsDepositorInsertPTK2 (PTK2 Mouse)
UseTagsmEGFPExpressionMammalianMutationPromotercmvAvailable sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-H3
Plasmid#109381PurposeArabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coliDepositorInsertpARABAD-VioB-mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti_SaCRISPR_GFP
Plasmid#118636PurposeTo clone sgRNA for SaCas9 (SauCas9)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterU6Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Sec24D
Plasmid#32678DepositorInsertSec24D (SEC24D Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
MF82_PB-TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
Plasmid#176626PurposeTF A self-activation cassette in MultiFate-2.2 and MultiFate-3DepositorInsertTRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEThT-SAHS2
Plasmid#90026PurposeTo express His-tagged SAHS2 in bacteriaDepositorInsertSAHS2
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV-Blast mLST8-Flag
Plasmid#124915PurposeFor stable expression sgRNA resistant Flag tagging mLST8_DepositorInsertMLST8 (MLST8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationInsert ORF was sirently mutated to be resistent t…PromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
LAMP-CeNL_pcDNA3
Plasmid#89514PurposeCyan color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1DepositorInsertCeNL
UseTagsLAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTK_Sox9_enh_349_Citrine
Plasmid#130594Purposeenhancer reporterDepositorInsertSox9_enh_349
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-Cdc42-2G
Plasmid#68814PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)DepositorInsertCdc42 activation reporter
UseTagsHis and MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cx36-GCaMP
Plasmid#123604PurposeMouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cellsDepositorInsertConnexin 36-GCaMP3 fusion (Gjd2 Mouse)
UseTagsGCaMP3ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-Lck-GFP
Plasmid#118738PurposeTo express membrane targeted GFPDepositorInsertLck domain (LCK Human)
UseLentiviralTagsGFPExpressionMutationH8N (see comments)PromoterCMVAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro-CAG-GCaMP6s
Plasmid#120896PurposeDonor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.DepositorInsertGCaMP6s
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX601-mCherry
Plasmid#84039PurposeStaphylococcus aureus (SaCas9) conjugated with mCherryDepositorInsertSaCas9
UseAAV and CRISPRTagsNLS and T2A-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
BDD FVIII H3
Plasmid#46775PurposepcDNA4.BDD FVIII.H3(M2238V)DepositorInsertB-domain deleted FVIII H3 protein (M2238V) (F8 Human)
UseTagsExpressionMammalianMutationM2238VPromoterCMVAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRG207
Plasmid#64530PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF3239
Plasmid#144715PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHOXB5 (HOXB5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Intron-Tagging-pX330-Cas9-Blast
Plasmid#159741PurposeExpresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.DepositorInsertdonor plasmid targeting sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-14-3-3 epsilon
Plasmid#116886PurposeExpresses HA-tagged human 14-3-3 epsilon in mammalian cellsDepositorInsert14-3-3 epsilon (YWHAE Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
MF83_PB-TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
Plasmid#176627PurposeTF B self-activation cassette in MultiFate-2.2 and MultiFate-3DepositorInsertTRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6b:sgRNA#3
Plasmid#64247Purposeexpresses sgRNA under U6b promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
hSirt3-RFP
Plasmid#216806PurposeHuman Sirt3-RFP driven by the CaMKII promoterDepositorInsertSirt3 (SIRT3 Human)
UseLentiviralTagsmRFP1ExpressionMammalianMutationPromoterCamKIIAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Carl-ReNL-KDEL_pcDNA3
Plasmid#89535PurposeRed color variant of bright luminescent protein, targeted to the endoplasmic reticulumDepositorInsertReNL
UseTagsCalreticulin and KDELExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pJL1-sfGFP
Plasmid#102634PurposeIn vitro expression of sfGFP from the T7 promoterDepositorInsertsfGFP
UseTagsstrep tagExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-lyso-pHoenix
Plasmid#70112PurposeExpression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomesDepositorInsertlyso-pHoenix (Cd63 Mouse, Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria)
UseTagsArch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-CMV-TO-Casp3te
Plasmid#116055PurposeReporter plasmid for measuring protease activityDepositorInsertCasp3te
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRyl
Plasmid#70007PurposeCRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertionDepositorInsertsCodon optimized Cas9 from S. pyogenes
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNCS mOrange
Plasmid#91764PurposeBacterial expression of a fluorescent protein, monomeric Orange (mOrange)DepositorInsertmonomeric Orange
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLG327
Plasmid#128797PurposeXylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.DepositorInsertgfpmut2 (NEWENTRY Synthetic)
UseTagsExpressionBacterialMutationPromoterPxyl(s)Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL18
Plasmid#183390PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertpro-IL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cox8x2-YeNL_pcDNA3
Plasmid#89517PurposeYellow color variant of bright luminescent protein, targeted to the MitochondriaDepositorInsertYeNL
UseTagsCoxVIII X2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
HBx
Plasmid#65463Purposeexpresses HBx in mammalian cellsDepositorInsertHBV X protein
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-BioID2-BioID2-HA
Plasmid#176855PurposeExpresses the promiscuous small biotin ligase, BioID2, in neuronsDepositorInsertBioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Carl-YeNL-KDEL_pcDNA3
Plasmid#89516PurposeYellow color variant of bright luminescent protein, targeted to the endoplasmic reticulumDepositorInsertYeNL
UseTagsCalreticulin and KDELExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbS5a-Opto-Cre-Vvd-2
Plasmid#160400PurposeUpdated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.DepositorInsertOpto-Cre-Vvd-2
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cox8x2-OeNL_pcDNA3
Plasmid#89526PurposeOrange color variant of bright luminescent protein, targeted to the MitochondriaDepositorInsertOeNL
UseTagsCoxVIII X2ExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pAc5.1B-EGFP
Plasmid#21181DepositorInsertenhanced green flourescent protein
UseDrosophila expressionTagsExpressionMutationPromoterAvailable sinceJune 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCCL/IFNB1-d2eGFP-3’UTR
Plasmid#180232PurposeReporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1DepositorInsertd2eGFP
UseLentiviralTagsExpressionMutationPromoter1 kb upstream of IFNB1 CDSAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Carl-CeNL-KDEL_pcDNA3
Plasmid#89509PurposeCyan color variant of bright luminescent protein, targeted to the endoplasmic reticulumDepositorInsertCeNL
UseTagsCalreticulin and KDELExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-HA-hTAF6
Plasmid#17988DepositorInsertTAF6 (TAF6 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Bsd-CMV-MDM4
Plasmid#202669PurposeMDM4 overexpression vector with blasticidin resistanceDepositorInsertMDM4 regulator of p53 (MDM4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human Erbb2-IRES-GFP
Plasmid#91888PurposeRetroviral expression of human Erbb2DepositorInsertErbb2 (ERBB2 Human)
UseRetroviralTagsIRES-EGFPExpressionMammalianMutationPromoterMMLV gagAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABE zeo PPP2CA Wt
Plasmid#10689DepositorInsertprotein phosphatase 2, catalytic subunit, alpha isozyme (PPP2CA Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC22A18_STOP
Plasmid#161213PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC22A18 (SLC22A18 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits