pBOB-Lck-GFP
(Plasmid
#118738)
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PurposeTo express membrane targeted GFP
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 118738 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCSC-SP-PW-GFP
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLck domain
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SpeciesH. sapiens (human)
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MutationH8N (see comments)
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Entrez GeneLCK (a.k.a. IMD22, LSK, YT16, p56lck, pp58lck)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xba I (unknown if destroyed)
- 3′ cloning site Age I (unknown if destroyed)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloned membrane targeting Lck sequence from popular Addgene plasmid #61099. H8N mutation does not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBOB-Lck-GFP was a gift from John Cooper (Addgene plasmid # 118738 ; http://n2t.net/addgene:118738 ; RRID:Addgene_118738)