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pcDNA Myc-mMEKK2

Plasmid

#44158

Depositor

Xin Lin

Article

Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

Insert

Mekk2 (Map3k2 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
FUW-ubiquitin-EphB2-SV40-GFP (B2G)

Plasmid

#65442

Purpose

Expression of mouse EPHB2 and GFP in mammalian cells

Depositor

Eduard Batlle

Article

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

EPHB2 (Ephb2 Mouse)

Use

Lentiviral

Tags

SV40-EGFP

Expression

Mammalian

Mutation

See depositor comments below.

Promoter

UBQ

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pBRIT Pax7 FLAG

Plasmid

#17521

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W

Plasmid

#67976

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AmAG cassette, WPRE

Use

Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKin1B

Plasmid

#31604

Depositor

Anthony Brown

Article

Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

Insert

kinesin-1B (Kif5b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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mLama1 AAV sgRNA 1, 2, 5

Plasmid

#135339

Purpose

Expression plasmid of VP64-SadCas9 sgRNA 1, 2 and 3

Depositor

Ronald Cohn

Article

Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

Insert

Lama1 VP64-dCas9 sgRNA Guides

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-CaMKKbeta

Plasmid

#32453

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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TNXXL-CTS20

Plasmid

#48342

Purpose

FRET reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

CTS20

Use

Tags

TN-XXL

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pORANGE GFP-CACNA1A KI

Plasmid

#131480

Purpose

Endogenous tagging of CaV2.1, P/Q: N-terminal (amino acid position: G10)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacna1a, Cacna1a Mouse, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRVL-3

Plasmid

#104581

Purpose

Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions

Depositor

Daniel Christ

Article

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Mouse IgG2c CH1/CH2/CH3 (mutated, no effector functions)

Use

Tags

Expression

Mammalian

Mutation

L234A/L235E/G237A and E318A/K320A/K322A

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Ttr:Cre

Plasmid

#32606

Depositor

Anna-Katerina Hadjantonakis

Article

Kwon et al Genesis. 2009 Jul;47(7):447-55.

Insert

TTR:Cre

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
Anti-Ankyrin-R [N388A/10R]

Plasmid

#114479

Purpose

Mammalian Expression Plasmid of anti-Ankyrin-R (Human). Derived from hybridoma N388A/10.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Ankyrin-R (Homo sapiens) recombinant mouse monoclonal antibody (ANK1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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qz5

Plasmid

#25867

Depositor

Bruce Conklin

Article

Conklin et al Nature. 1993 May 20. 363(6426):274-6.

Insert

Gq alpha (Gnaq Mouse)

Use

Tags

HA (see comments)

Expression

Mammalian

Mutation

Replacement of the five carboxyl-terminal amino a…

Promoter

Available since

Aug. 25, 2010

Availability

Academic Institutions and Nonprofits only

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Nav1.7 Na+ channel scFv [N68/6]

Plasmid

#182094

Purpose

Mammalian Expression of Nav1.7 Na+ channel scFv. Derived from hybridoma N68/6.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Nav1.7 Na+ channel (Homo sapiens) (SCN9A Mouse)

Use

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Src-EGFP

Plasmid

#110496

Purpose

Fluorescent Src

Depositor

Terence Dermody, Bernardo Mainou

Article

Mainou et al J Virol. 2011 Apr;85(7):3203-13. doi: 10

Insert

c-Src (Src Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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5HT6-YC3.60

Plasmid

#48339

Purpose

FRET reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

5-hydroxytriptamine receptor isoform 6 (Htr6 Mouse)

Use

Tags

YC3.60

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
KA2628_pGL4-UBCmin-luc2/Tbx3-B'

Plasmid

#124201

Purpose

Luciferase reporter driven by the Tbx3 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Tbx3 enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV PIG C/EBPa

Plasmid

#66985

Purpose

Retroviral expression plasmid for mouse C/EBPa expression; containing bicistronic IRES-GFP

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

C/EBP alpha (Cebpa Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

LTR

Available since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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Anti-Cav3.1 Ca2+ channel [N178A/9R-2b]

Plasmid

#206660

Purpose

Mammalian Expression Plasmid of anti-Cav3.1 Ca2+ channel (Mouse). Derived from hybridoma N178A/9-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Cav3.1 Ca2+ channel (Mus musculus) recombinant Mouse monoclonal antibody (Cacna1g Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCI pHluorin Syt10

Plasmid

#184511

Purpose

Mammalian expression of Synaptotagmin 10 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 10 (Syt10 Mouse)

Use

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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