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Plasmid#167935PurposeInducible CRISPRi expressionDepositorInsertKRAB-dCas9-DHFR
UseLentiviralTagsExpressionMutationPromotertetONAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti-NLS-mTagBFP2
Plasmid#216128PurposeLentiviral vector for expressing mTagBFP2 localizing to the nucleusDepositorInsertEF1a-NuclearLocalizationSignal-mTagBFP2-WPRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Luciferase-pcDNA3
Plasmid#18964DepositorInsertFirefly Luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDuet TDP43 WT
Plasmid#27462DepositorInsertTDP-43 (TARDBP Human)
UseCoexpressionTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP713-N1
Plasmid#136559PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP713DepositorInsertmiRFP713
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
psfTn5
Plasmid#79107Purposebacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutationsDepositorInserttransposase 5
UseTagsHisExpressionBacterialMutationE54K and L372PPromoterAvailable sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-ER-5
Plasmid#55294PurposeLocalization: Endoplasmic Reticulum, Excitation: 399, Emission: 456DepositorInsertER (CALR Human)
UseTagsmTagBFP2ExpressionMammalianMutationER Signal PeptidePromoterCMVAvailable sinceOct. 7, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF3450
Plasmid#144926PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertELK1 (ELK1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3550
Plasmid#145026PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.DepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMF230
Plasmid#62546PurposeBroad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.DepositorInsertGFP mut2
UseBroad host range plasmidTagsExpressionMutationPromotertrcAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
7b sgRNA for EJ7-GFP reporter
Plasmid#113624PurposesgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporterDepositorInsert7b sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
7a sgRNA for EJ7-GFP and 4-μHOM reporters
Plasmid#113620PurposesgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reportersDepositorInsert7a sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_Ado1.0med
Plasmid#153286PurposeExpress the medium-affinity adenosine sensor GRAB_Ado1.0med in neuronsDepositorInsertAdenosine sensor GRAB_Ado1.0med (medium affinity)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1180 U6-reci Gag-pol v2
Plasmid#201915PurposeCas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)DepositorInsertGag-pol
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_MET_3Flag
Plasmid#182496PurposeContains cDNA of wt METDepositorInsertMET (MET Human)
UseTagsC-terminal 3xFlag tagExpressionMammalianMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGolden-AAV
Plasmid#51424PurposeContain AAV ITRs, lacZ expressing for blue-white selection for golden-gate reactionDepositorInsertlacZ
UseAAVTagsExpressionMutationPromoterlacZAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFB2E
Plasmid#195288PurposeBicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selectionDepositorInsertHSV-TK EGFP 2A Puro
UseTagsExpressionMammalianMutationPromoterEFSAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-STAT6-IRES-Neo
Plasmid#35482DepositorInsertSTAT6 (STAT6 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLEX_305-C-dTAG
Plasmid#91798PurposeLentiviral/gateway cloning vector for C-terminally tagging proteins of interest with the dTAG systemDepositorTypeEmpty backboneUseLentiviralTagsFKBP F36VExpressionMammalianMutationPromoterhPGKAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huBsCas12a
Plasmid#174671PurposeExpresses 6xHis-MBP tagged BsCas12aDepositorArticleInserthuBsCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…ExpressionBacterialMutationPromoterT7Available sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only