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PurposeMammalian expression of ultrafast protein calcium sensor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 162371 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerThermoFisher Invitrogen
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namejGCaMP8s
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Alt namevariant 688
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SpeciesR. norvegicus (rat), G. gallus (chicken); A. victoria (jellyfish)
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Insert Size (bp)1269
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MutationS26M F286Y Q315H
- Promoter CMV
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGP-CMV-jGCaMP8s was a gift from GENIE Project (Addgene plasmid # 162371 ; http://n2t.net/addgene:162371 ; RRID:Addgene_162371) -
For your References section:
jGCaMP8 Fast Genetically Encoded Calcium Indicators. Zhang Y, Rózsa M, Bushey D, Zheng J, Reep D, Broussard GJ, Tsang A, Tsegaye G, Patel R, Narayan S, Li JX, Zhang R, Ahrens MB, Turner GC, Wang SS-H, Svoboda K, Korff W, Schreiter ER, Hasseman JP, Kolb I, Looger LL. Janelia Research Campus, Online Resource (2020) doi:10.25378/janelia.13148243. 10.25378/janelia.13148243