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Plasmid#163770PurposeExpression of CARD domain variant with a deletion of exon 3DepositorInsertCARD11 (CARD11 Human)
UseLentiviralTagsExpressionMutationDel exon 3PromoterMNDAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFD6
Plasmid#73915Purposetissue-specific multiplex gRNA plasmidDepositorInsert:tRNA:gRNA:tRNA:gRNA:tRNA
UseCRISPRTagsExpressionInsectMutationPromoterUAS-DSCPAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_MP02
Plasmid#194351PurposeCRISPR-Cas9 multiplexing acceptor clone with BsaI cutterDepositorInsertpcoCas9
UseTagsExpressionPlantMutationPromoterRPS5AAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKIIa(0.4)-stChrimsonR-EGFP-P2A-PdCO-WPRE
Plasmid#202198PurposeExpresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of minimal CamKIIa promotorDepositorInsertstChrimsonR, PdCO
UseAAVTagsEGFP and Rho1D4ExpressionMammalianMutationPromoterCaMKIIα minimal promotor (0.4kb)Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE
Plasmid#202199PurposeExpresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombinationDepositorHas ServiceAAV1 and AAV5InsertstChrimsonR, PdCO
UseAAV and Cre/LoxTagsEGFP and Rho1D4ExpressionMammalianMutationPromoterhSyn1Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYZ038
Plasmid#200227Purposeuniversal template to build tRNA-gRNA array for multiplex editing CREEPYDepositorInserttRNA-gRNA scaffold
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_MP01
Plasmid#194350PurposeCRISPR-Cas9 multiplexing acceptor clone with BpiI cutterDepositorInsertpcoCas9, FAST-Red
UseTagsExpressionPlantMutationPromoterRPS5AAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-16
Plasmid#104966PurposePlasmid expressing two multiplexed ribozyme-gRNA-ribozyme (RGR) gRNAsDepositorInsertRibozyme-gRNA-ribozyme
UseCre/LoxTagsExpressionInsectMutationPromoterAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSQT1313
Plasmid#53370Purposemultiplex gRNA expression plasmidDepositorInsertNone
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbOcIgG-Tn5
Plasmid#184285PurposeAnti-rabbit IgG Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbOcIgG-Tn5
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmKappa-Tn5
Plasmid#184286PurposeAnti-mouse kappa chain specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmKappa-Tn5
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmIgG1-Tn5
Plasmid#184287PurposeAnti-mouse IgG1 Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmIgG1-Tn5
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmIgG2a-Tn5
Plasmid#184288PurposeAnti-mouse IgG2a Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmIgG2a-Tn5
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGE4
Plasmid#79443Purposerecipient plasmid [multiplexing]; pPcUbi:Cas9, BASTADepositorInsertccdB (ccdB(letD) )
UseCRISPRTagsExpressionPlantMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE3
Plasmid#79442Purposerecipient plasmid [multiplexing]; pPcUbi:Cas9, nptIIDepositorInsertccdB (ccdB(letD) )
UseCRISPRTagsExpressionPlantMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE1
Plasmid#79440Purposerecipient plasmid [multiplexing]; p35S:Cas9, nptIIDepositorInsertccdB (ccdB(letD) )
UseCRISPRTagsExpressionPlantMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
Pub-mCD8:GFP-T2A-DsRed:NLS-SV40
Plasmid#130665PurposePlasmid to test the efficiency of T2A multiplexing in Aedes aegyptiDepositorInsertAaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40
UseAe. aegypti expressionTagsExpressionInsectMutationPromoterAedes aegypti poly-ubiquitinAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGE146
Plasmid#79449Purposerecipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, BASTADepositorInsertccdB (ccdB(letD) )
UseCRISPRTagsExpressionPlantMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE167
Plasmid#79457Purposerecipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, HygroDepositorInsertccdB (ccdB(letD) )
UseCRISPRTagsExpressionPlantMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE168
Plasmid#79458Purposerecipient plasmid [multiplexing]; pDD45:Cas9, FAST, HygroDepositorInsertccdB (ccdB(letD) )
UseCRISPRTagsExpressionPlantMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only