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Purposemultiplex gRNA expression plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 53370 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMLM3636
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Backbone manufacturerJoung lab (Addgene plasmid # 43860)
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
- Promoter U6
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer OS280 (5’-CAGGGTTATTGTCTCATGAGCGG-3’) (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSQT1313 was a gift from Keith Joung (Addgene plasmid # 53370 ; http://n2t.net/addgene:53370 ; RRID:Addgene_53370) -
For your References section:
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908. 10.1038/nbt.2908 PubMed 24770325