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Plasmid#71666PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, Human, S. pyogenes)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-EGFPExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VSVL
Plasmid#64085PurposeExpression vector for Vesicular Stomatitis Virus Indiana strain polymerase geneDepositorInsertVSV polymerase
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUmGW
Plasmid#22479DepositorInsertmembrane-bound green fluorescent protein
UseLentiviralTagspalmitoylationExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGL2-NOS2Promoter-Luciferase
Plasmid#19296DepositorInsertNOS2 Promoter (Nos2 Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDest eGFP MBNL1 42 kDa
Plasmid#61277PurposeMammalian expression of EGFP-tagged human MBNL1DepositorInsertMBNL1 (MBNL1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV rtTA3 Hygro (w785-1)
Plasmid#26730Purpose3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, HygroĀDepositorInsertTetracycline repressor A3 mutant
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-DsRed
Plasmid#11151PurposeMammalian expression vector for expression of DsRedDepositorHas ServiceCloning Grade DNAInsertDsRed2 from Discosoma sp
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pU6a:sgRNA#2
Plasmid#64246Purposeexpresses sgRNA under U6a promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-R-GECO1
Plasmid#32444PurposeMammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imagingDepositorArticleInsertR-GECO1.0
UseTagsExpressionMammalianMutationSubstitutions relative to the mApple-derived analā¦PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDESTmycDDX17
Plasmid#19876DepositorInsertDEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17 Human)
UseTagsMYCExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro GFP-LC3
Plasmid#22405Purposeretroviral expression of GFP-tagged LC3DepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMDIAI
Plasmid#51655PurposeE coli genetic modificationDepositorInsertapramycin antibiotic resistance gene
UseGenetic modificationTagsExpressionMutationPromoterunknownAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-IRES-Neo
Plasmid#85139Purposelentiviral backboneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-n1-APP
Plasmid#69924Purposemammalian expression of human APP fused to EGFPDepositorInsertAPP (APP Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
NLS-mCherry-LEXY (pDN122)
Plasmid#72655PurposeBlue light-inducible nuclear export of mCherryDepositorInsertNLS-mCherry-LEXY
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti_gRNA-Puro
Plasmid#84752PurposeExpresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6
Plasmid#39296DepositorInsertKanR
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a geneā¦ExpressionMutationPromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pC1-HyPer-3
Plasmid#42131DepositorInsertHYPER 3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP670-N1
Plasmid#79987PurposeMonomeric near-infrared fluorescent protein miRFP670 in mammalian plasmidDepositorHas ServiceCloning Grade DNAInsertmiRFP670
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
MPZ(P0)promoter pGL3-TATA
Plasmid#21259DepositorInsertmyelin protein zero promoter (Mpz Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2009AvailabilityAcademic Institutions and Nonprofits only