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Showing: 5201 - 5240 of 7539 results

  3. pKK-mRuby3-TEV

    Plasmid
    #105780
    Purpose
    Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mRuby3-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105780-plasmid-map-sequence-id-203369

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  4. pAAV.hSynapsin.SF-iGluSnFR.S72A

    Plasmid
    #106176
    Purpose
    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Has Service
    AAV1
    Insert
    SF-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: S72A
    Promoter
    hSynapsin
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    106176-plasmid-map-sequence-id-213318

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  6. pCW-GemininΔDbox-HA-Puro

    Plasmid
    #193770
    Purpose
    Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    GemininΔDbox-HA (GMNN Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Dbox removed (R23A, L26A). Codons switched at siR…
    Promoter
    TRE
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193770-plasmid-map-sequence-id-380213

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  9. pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

    Plasmid
    #207805
    Purpose
    E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.
    Depositor
    Koen Breyne
    Article
    Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do
    Insert
    E-NoMi
    Use
    Lentiviral
    Tags
    3xFlag tag, copGFP, mCherry, and nanoluciferase
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1alpha
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    207805-plasmid-map-sequence-id-419158

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  12. pC-G418-YR

    Plasmid
    #61767
    Purpose
    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).
    Depositor
    Ken Haynes
    Article
    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do
    Inserts
    nptII gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    Use
    Ternary vector for agrobacterium mediated transfo…
    Tags
    Expression
    Mutation
    Promoter
    trpC promoter
    Available since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    61767-plasmid-map-sequence-id-367929

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  17. LentiRCas9-Lambda2

    Plasmid
    #104184
    Purpose
    Lentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)
    Depositor
    Eugene Yeo
    Article
    Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do
    Insert
    U6-sgRNA, EFS-PIN-dCas9
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    104184-plasmid-map-sequence-id-201745

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  18. pKan cpRBP-FL P53

    Plasmid
    #174504
    Purpose
    Express Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)
    Depositor
    Stewart Loh
    Article
    Ha et al Bio Protoc. 2021 Oct 20;11(20):e4188. do
    Insert
    P53 gene (TP53 Human)
    Use
    Tags
    20 amino acid linker containing HRV3C protease si…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174504-plasmid-map-sequence-id-344828

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  25. pET28a-CTLA4-FLN-ELP-His-ybbr

    Plasmid
    #168038
    Purpose
    Expresses CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus
    Depositor
    Michael Nash
    Article
    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do
    Insert
    Cytotoxic T-lymphocyte associated protein 4 (CTLA4 Human)
    Use
    Tags
    3x ELP linker, 6x Histag, ddFLN4 (C18S), and ybbr…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168038-plasmid-map-sequence-id-334767

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  34. pSin-EF2-DMD53-GFP

    Plasmid
    #138353
    Purpose
    Expressing a GFP reporter cassette, whose GFP reading frame is disrupted by insertion of partial DMD exon 53 sequences
    Depositor
    Baisong Lu
    Article
    Lyu et al PLoS One. 2020 Sep 24;15(9):e0239468. do
    Insert
    DMD exon 53 partial sequence to disrupt GFP reading frame (DMD Human)
    Use
    CRISPR and Lentiviral
    Tags
    No
    Expression
    Mammalian
    Mutation
    Promoter
    EF2
    Available since
    Nov. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    138353-plasmid-map-sequence-id-273452

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  35. pKK-TEV-mRuby3

    Plasmid
    #105797
    Purpose
    Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mRuby3
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105797-plasmid-map-sequence-id-203388

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Showing: 5201 - 5240 of 7539 results