pSin-EF2-DMD53-GFP
(Plasmid #138353)

• Purpose: Expressing a GFP reporter cassette, whose GFP reading frame is disrupted by insertion of partial DMD exon 53 sequences
• Depositing Lab: Baisong Lu
• Publication: Lyu et al PLoS One. 2020 Sep 24;15(9):e0239468. doi: 10.1371/journal.pone.0239468. eCollection 2020.

Backbone

• Vector backbone: pSin-EF2-IL2-HHB-GFP reporter
• Backbone manufacturer: Lu Lab
• Backbone size w/o insert (bp): 8241
• Total vector size (bp): 8315
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DMD exon 53 partial sequence to disrupt GFP reading frame
• Species: H. sapiens (human)
• Insert Size (bp): 74
• GenBank ID: NM_000109.4
• Entrez Gene: DMD (a.k.a. BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, MRX85)
• Promoter: EF2
• Tag / Fusion Protein:
  • No

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: No
• 3′ sequencing primer: No

Resource Information

• Supplemental Documents:
  • 138353-attachment_w6sWwpwXwpfDo8OLwp7Djw.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry