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Plasmid#124423PurposeFull MCS (step 2)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only
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pAAV-hSyn-JEDI-2P-WPRE
Plasmid#179464PurposeGenetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSynDepositorHas ServiceAAV9InsertJEDI-2P
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-JEDI-2P
Plasmid#179458PurposeGenetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)DepositorInsertJEDI-2P
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Ef1a-mClover-W377A/W428A YTHDC1-T2A-BSD-siRNA-Resistant
Plasmid#177130PurposeGenerate lentivirus to introduce mClover tagged m6A binding null mutant W377A/W428A YTHDC1DepositorInsertYTHDC1 (YTHDC1 Human)
UseLentiviralTagsmClover3ExpressionMutationW428A/W377A (m6A binding defects), and siRNA resi…PromoterEf1aAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE
Plasmid#104495PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switchDepositorHas ServiceAAV PHP.eB, AAV Retrograde, and AAV1InsertjGCaMP7s
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCAGAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-JEDI-2P-WPRE
Plasmid#179469PurposeGenetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for astrocytes specific expression using the promoter GFAPDepositorInsertJEDI-2P
UseAAVTagsExpressionMammalianMutationPromoterGFAPAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-JEDI-2P-WPRE
Plasmid#179466PurposeGenetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKIIDepositorInsertJEDI-2P
UseAAVTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
plenti-ef1a-dCasRx-FTO-HA-T2A-BSD
Plasmid#177120PurposedCasRx mediated RNA tethering of FTODepositorInsertdCasRx-FTO
UseLentiviralTagsHA and SV40 NLSExpressionMutationdCasRX contains R239A/H244A/R858A/H863A mutations…PromoterEf1aAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE
Plasmid#105322PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre-dependent expressionDepositorHas ServiceAAV1InsertjGCaMP7c variant 1513
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterSynapsinAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
px330-Pten-2
Plasmid#162552PurposesgRNA targeting PtenDepositorInsertsgRNA targeting Pten (Pten Mouse)
UseTagsExpressionMammalianMutationNonePromoterU6Available sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP7c variant 1513-WPRE
Plasmid#105323PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre-dependent expressionDepositorInsertjGCaMP7c variant 1513
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCAGAvailable sinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-JEDI-2P
Plasmid#179461PurposeGenetically encoded voltage indicator (GEVI) JEDI-2P under the promoter hsp70 for Drosophila (insect) expressionDepositorInsertJEDI-2P
UseTagsExpressionInsectMutationPromoterhsp70Available sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
px330-Apob-2
Plasmid#162549PurposesgRNA targeting ApobDepositorInsertsgRNA targeting Apob (Apob Mouse)
UseTagsExpressionMammalianMutationNonePromoterU6Available sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330-Apob-1
Plasmid#162548PurposesgRNA targeting ApobDepositorInsertsgRNA targeting Apob (Apob Mouse)
UseTagsExpressionMammalianMutationNonePromoterU6Available sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330-Apob-3
Plasmid#162550PurposesgRNA targeting ApobDepositorInsertsgRNA targeting Apob (Apob Mouse)
UseTagsExpressionMammalianMutationNonePromoterU6Available sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330-Apob-4
Plasmid#162551PurposesgRNA targeting ApobDepositorInsertsgRNA targeting Apob (Apob Mouse)
UseTagsExpressionMammalianMutationNonePromoterU6Available sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pTXBI-mClover-YTH domain
Plasmid#177123PurposeBacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purificationDepositorInsertYTHDC1 (YTH domain) (YTHDC1 Human)
UseTagsMxe GyrA intein-Chitin binding domain for protein…ExpressionBacterialMutationYTH domain only (IDR1 and IDR2 deletion)PromoterT7Available sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXBI-mclover-YTHDC1-FL
Plasmid#177121PurposeBacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purificationDepositorInsertYTHDC1 (YTHDC1 Human)
UseTagsMxe GyrA intein-Chitin binding domain for protein…ExpressionBacterialMutationPromoterT7Available sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXBI-mclover-YTH-IDR2
Plasmid#177122PurposeBacterial expression of YTH-IDR2 fragment of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purificationDepositorInsertYTHDC1 (YTH and IDR2 domains) (YTHDC1 Human)
UseTagsMxe GyrA intein-Chitin binding domain for protein…ExpressionBacterialMutationYTH and IDR2 domains only (IDR1 deletion)PromoterT7Available sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only