pTXBI-mClover-YTH domain
(Plasmid #177123)

• Purpose: Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
• Depositing Lab: Wenbo Li
• Publication: Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e9. doi: 10.1016/j.molcel.2021.07.024. Epub 2021 Aug 9.

Backbone

• Vector backbone: Addgene 61425
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YTHDC1 (YTH domain)
• Species: H. sapiens (human)
• Mutation: YTH domain only (IDR1 and IDR2 deletion)
• Entrez Gene: YTHDC1 (a.k.a. YT521, YT521-B)
• Promoter: T7
• Tags / Fusion Proteins:
  • mClover3 (N terminal on insert)
  • Mxe GyrA intein-Chitin binding domain for protein purification (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pTXB1-mClover-YTHDC1-YTH domain.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains YTH-domain isoform 14. Plasmid also contains an E14K mutation in Mxe GyrA intein, which is not known to affect plasmid function.