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pGL3-Basic-IRES

Plasmid

#64784

Purpose

Luciferase reporter vector

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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pLOX-Ttag-iresTK

Plasmid

#12246

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

SV40 Large T, HSV1-TK (SV40gp6 Simian virus)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-SHP2WT

Plasmid

#12283

Depositor

Anton Bennett

Article

Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

Insert

SHP-2WT (PTPN11 Human)

Use

Tags

Myc (not canonical: KLISEEDL)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pIRES2-smGFP-Kv2.1

Plasmid

#131709

Purpose

Expresses Kv2.1 in mammalian cells

Depositor

Jennifer Kearney

Article

Kang et al Ann Neurol. 2019 Dec;86(6):899-912. doi:

Insert

KCNB1 (KCNB1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pirespuro2-CTLA4-mEos2

Plasmid

#98285

Purpose

mammalian expression of CTLA4-mEos2

Depositor

Mike Heilemann

Article

Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

Insert

CTLA4 (CTLA4 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-Ki-67(RASA)-mCherry_IRESpuro2

Plasmid

#182921

Purpose

Expresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.

Depositor

Daniel Gerlich

Article

Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

Insert

MKI67 (MKI67 Human)

Use

Tags

EGFP and mCherry

Expression

Bacterial and Mammalian

Mutation

RASA mutation

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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p27-IRES-mCherry

Plasmid

#80143

Purpose

retroviral expression of p27 and mCherry

Depositor

Ellen Rothenberg

Article

Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

Insert

p27 (CDKN1B Human)

Use

Retroviral

Tags

IRES-mCherry

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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DD-DamV133A-IRES2-mCherry

Plasmid

#159600

Purpose

Shield-1 inducible transient mammalian expression of Dam alone for DamID controls, containing the V133A mutant of Dam.

Depositor

Aaron Streets

Article

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

DD-DamV133A-IRES2-mCherry

Use

Tags

Expression

Mammalian

Mutation

Dam V133A mutation

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pIRES2-hPAC-FLAG

Plasmid

#129494

Purpose

Expresses human TMEM206 tagged at the C-terminus with FLAG

Depositor

Zhaozhu Qiu

Article

Yang et al Science. 2019 Apr 26;364(6438):395-399.

Insert

human PAC tagged with FLAG tag at the C-terminal end (PACC1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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TRF2-IRESS-eGFP

Plasmid

#19798

Depositor

Mark Mattson

Article

van Steensel et al Cell. 1998 Feb 6. 92(3):401-13.

Insert

TRF2 (TERF2 Human)

Use

Tags

Expression

Mammalian

Mutation

When compared to GenBank reference sequence NP_00…

Promoter

CMV-F

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
Ki67 (LR)-mNeonGreen-IRESpuro2

Plasmid

#183743

Purpose

Expresses Ki67 (LR)-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 LR domain (MKI67 Human)

Use

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

LR domain of Ki67

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pB-Halo,IRES-eGFP

Plasmid

#164519

Purpose

All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLVET-IRES-GFP

Plasmid

#107139

Purpose

A bicistronic lentiviral mammalian expression vector with a multiple cloning site and GFP

Depositor

Aki Manninen

Article

Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLPCX-IRES-GFP

Plasmid

#65436

Purpose

For transfection or retroviral production. Mammalian expression of EGFP driven by the ECMV IRES.

Depositor

Trond Aasen

Article

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

IRES-GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#112924

Purpose

piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo

Use

Piggybac transposon vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX_BHLHE40-FlagHA_IRES-ZsGreen1

Plasmid

#149714

Purpose

Lentiviral vector expressing human BHLHE40 with FLAG-HA tags

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

BHLHE40 (BHLHE40 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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piRES-puro-ELF3

Plasmid

#25728

Depositor

Ronny Drapkin

Insert

E74-like factor 3 (ets domain transcription factor, epithelial-specific ) (ELF3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pKH151_PB_flag-LC3B_wt_IRES-BFP

Plasmid

#214976

Purpose

Flag tagged LC3B

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-LC3B (MAP1LC3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pHR V5-RNF25_IRES-mCherry

Plasmid

#198386

Purpose

RNF25 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RNF25 (RNF25 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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SiRES COR1C-Halo

Plasmid

#195153

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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